Single-cell transcriptomic profile of satellite glial cells in trigeminal ganglion

Frontiers in Molecular Neuroscience

Molecular Neuroscience

Lijia Mai¹  Wenguo Fan¹  Jiawei Liu¹  Shilin Jia¹  Fang Huang¹  Yanhao Chu¹  Ke Xu¹  Qing Liu² 

[1] Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China;Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China;Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China;Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong SAR, China;

关键词: single-cell RNA sequencing;    trigeminal ganglion;    satellite glial cell;    cell heterogeneity;    neuron-glia communication;   

DOI  :  10.3389/fnmol.2023.1117065

 received in 2022-12-06, accepted in 2023-01-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

Satellite glial cells (SGCs) play an important role in regulating the function of trigeminal ganglion (TG) neurons. Multiple mediators are involved in the bidirectional communication between SGCs and neurons in different physiological and pathological states. However, molecular insights into the transcript characteristics of SGCs are limited. Moreover, little is known about the heterogeneity of SGCs in TG, and a more in-depth understanding of the interactions between SGCs and neuron subtypes is needed. Here we show the single-cell RNA sequencing (scRNA-seq) profile of SGCs in TG under physiological conditions. Our results demonstrate TG includes nine types of cell clusters, such as neurons, SGCs, myeloid Schwann cells (mSCs), non-myeloid Schwann cells (nmSCs), immune cells, etc., and the corresponding markers are also presented. We reveal the signature gene expression of SGCs, mSCs and nmSCs in the TG, and analyze the ligand-receptor pairs between neuron subtypes and SGCs in the TG. In the heterogeneity analysis of SGCs, four SGCs subtypes are identified, including subtypes enriched for genes associated with extracellular matrix organization, immediate early genes, interferon beta, and cell adhesion molecules, respectively. Our data suggest the molecular characteristics, heterogeneity of SGCs, and bidirectional interactions between SGCs and neurons, providing a valuable resource for studying SGCs in the TG.

【 授权许可】

Unknown   
Copyright © 2023 Chu, Jia, Xu, Liu, Mai, Liu, Fan and Huang.