Single Cell RNA Sequencing Reveals the Pathogenesis of Aortic Dissection Caused by Hypertension and Marfan Syndrome

Frontiers in Cell and Developmental Biology

Zhihuang Qiu¹  Xi Yang¹  Jian He¹  Liangwan Chen¹  Hui Zheng¹  Yumei Li²  Jianqiang Ye²  Li Zhang³ 

[1] Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China;Fujian Center for Safety Evaluation of New Drug, Fujian Medical University, Fuzhou, China;The Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases, The School of Basic Medical Sciences, Fuzhou, China;

关键词: aortic dissection;    single-cell RNA sequencing;    cell-cell communication;    cell heterogeneity;    RNA velocity;   

DOI  :  10.3389/fcell.2022.880320

来源: DOAJ

【 摘 要 】

Aortic dissection (AD) is mainly caused by hypertension and Marfan syndrome. However, it is unclear whether the cellular components and functions are different between the two causes. A total of 11 aortic samples were collected for single-cell RNA analysis and 20 clusters were disclosed, including VSMCs, fibroblasts, endothelial cells, T cells, B cells, monocytes, macrophages, mast cells, and neutrophils components. There were differences in cell subclusters and function between hypertension and Marfan patients. The cells also had different differentiations. Cellchat identified cell ligand–receptor interactions that were associated with hypertension and Marfan-induced AD involving SMC, fibroblast, mo-macrophages, and T-cell subsets. This study revealed the heterogeneity of cellular components and gene changes in hypertension and Marfan-induced AD. Through functional analysis and the changes in intercellular communication, the possible mechanisms of different causes of AD were explained from a new perspective, so we can better understand the occurrence and development of diseases.

【 授权许可】

Unknown