期刊论文详细信息
Frontiers in Immunology
Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis
Immunology
Huiling Tan1  Zhuochun Huang1  Ruyu Ren1  Bin Yang1  Yuanyi Wang2 
[1] Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China;Department of Spine Surgery, The First Hospital of Jilin University, Changchun, China;
关键词: piRNA;    PIWI;    rheumatoid arthritis;    peripheral blood;    biomarker;   
DOI  :  10.3389/fimmu.2023.1175924
 received in 2023-02-28, accepted in 2023-05-17,  发布年份 2023
来源: Frontiers
PDF
【 摘 要 】

BackgroundAlthough PIWI-interacting RNAs (piRNAs) have recently been associated with germline development and many human diseases, their expression pattern and relationship in autoimmune diseases remain indistinct. This study aimed to investigate the presence and correlation of piRNAs in rheumatoid arthritis (RA).MethodsWe first analyzed the expression profile of piRNAs using small RNA sequencing in peripheral leukocytes of three new-onset untreated RA patients and three healthy controls (HCs). We then selected piRNAs related to immunoregulation by bioinformatics analysis and verified them in 42 new-onset RA patients and 81 HCs by RT-qPCR. Furthermore, a receiver operating characteristic curve was generated to quantify the diagnostic performance of these piRNAs. A correlation analysis was conducted to observe the link between piRNA expression and RA clinical characteristics.ResultsA total of 15 upregulated and 9 downregulated piRNAs among 1,565 known piRNAs were identified in peripheral leukocytes of RA patients. Dysregulated piRNAs were enriched in numerous pathways related to immunity. After selection and validation, two immunoregulation piRNAs (piR-hsa-27620 and piR-hsa-27124) were significantly elevated in RA patients and have good abilities to distinguish patients from controls, which have the potential to serve as biomarkers. PIWI and other proteins implicated in the piRNA pathway were also associated with RA.

【 授权许可】

Unknown   
Copyright © 2023 Ren, Tan, Huang, Wang and Yang

【 预 览 】
附件列表
Files Size Format View
RO202310103031821ZK.pdf 4077KB PDF download
  文献评价指标  
  下载次数:4次 浏览次数:1次