The effects of fermented feedstuff derived from Citri Sarcodactylis Fructus by-products on growth performance, intestinal digestive enzyme activity, nutrient utilization, meat quality, gut microbiota, and metabolites of broiler chicken

Frontiers in Veterinary Science

Veterinary Science

Yilong Jiang¹  Huaidan Zhang¹  Chuanpeng Yang¹  Shiyi Li¹  Lijuan Kang¹  Jicheng Deng¹  Xin Zhao¹  Xianxin Chen¹  Jingjing Zhao²  Li Jiang³  Xinhong Zhou⁴ 

[1] Leshan Academy of Agriculture Science, Leshan, Sichuan, China;Leshan Animal Disease Prevention and Control Center, Leshan, Sichuan, China;School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China;School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China;Leshan Academy of Agriculture Science, Leshan, Sichuan, China;

关键词: broiler chickens;    fermented feed;    growth performance;    gut microbiota;    gut microbiota metabolites;   

DOI  :  10.3389/fvets.2023.1231996

 received in 2023-05-31, accepted in 2023-06-19,  发布年份 2023

来源: Frontiers

【 摘 要 】

This research aimed to assess the impact of fermented Citri Sarcodactylis Fructus by-products (FCSF) on the growth performance, gut digestive enzyme activity, nutrient utilization efficiency, gut microbiota, and their metabolites in broiler chickens. A total of 1,080 male broiler chickens were allocated into four groups (T1–T4) consisting of 6 replicates per group, each containing 45 chickens. The basal diet was provided to group T1, while groups T2, T3, and T4 were supplemented with 1%, 3%, and 5% FCSF in the basal diet, respectively. The experimental period was 42 days. The findings revealed that supplementing FCSF improved the FW and ADG of broiler chickens, and led to a reduction in the F/G, ADFI, and mortality rate of broiler chickens (p < 0.05). Furthermore, supplementation with 3% and 5% FCSF improved the thigh yield, semi-eviscerated carcass yield, slaughter yield, and lipase activity in the duodenum and ileum of birds (p < 0.05). Additionally, supplementing 3% FCSF enhanced the activity of protease in the duodenum of broilers (p < 0.05). Moreover, supplementing 3% FCSF enhanced the utilization of total phosphorus, dry matter, crude protein, and crude ash in the feed by broilers (p < 0.05). Compared with the control group, supplementation of 3% and 5% FCSF reduced the serine content in broiler chicken breast meat (p < 0.05). Supplementing 1% FCSF significantly increased the C14:0, C14:1, and C20:1 content in the breast meat compared to the other experimental groups (p < 0.05). The levels of C20:4n6 and C23:0 in the breast meat of birds of FCSF supplemented groups were lower than in T1 (p < 0.05). Furthermore, the content of ∑ω-3PUFA decreased after supplementing with 3% and 5% FCSF (p < 0.05). 16SrDNA showed that supplementing 3% FCSF reduced the ACE, Chao1, and Shannon indices in the cecum of birds (p < 0.05). Supplementing 3% FCSF also decreased the abundance of the phylum Desulfobacterota and improved genera Coprobacter and Prevotella in the cecum of broiler chickens (p < 0.05). Metabolomic analysis of the gut microbiota revealed that supplementing 3% FCSF upregulated 6 metabolites and downregulated 16 metabolites (p < 0.05). Moreover, supplementing 3% FCSF downregulated 12 metabolic pathways and upregulated 3 metabolic pathways (p < 0.05). In summary our findings indicate that supplementing FCSF can improve the growth performance of broiler chickens by enhancing intestinal digestive enzyme activity, nutrient utilization, improving gut microbial diversity, and influencing the metabolism of gut microbiota.

【 授权许可】

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Copyright © 2023 Zhou, Zhang, Li, Jiang, Kang, Deng, Yang, Zhao, Zhao, Jiang and Chen.