Developmental, Dietary, and Geographical Impacts on Gut Microbiota of Red Swamp Crayfish (Procambarus clarkii)

Microorganisms

Chao Liu¹  Jinshui Zheng²  Yunxiang Liang³  Jiali Liu³  Nan Peng³  Chenwei Fan³  Zhenting Zhang³  Xuexia Jin³  Li Guo⁴ 

[1] Runge College of Bioengineering, Sichuan Runge Biotechnology Co., Ltd., Mianzhu, Deyang 618200, China;State Key Laboratory of Agricultural Microbiology, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China;State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;State Key Laboratory of Electrical Insulation and Power Equipment, Center for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, China;

关键词: red swamp crayfish;    gut microbiota;    shaping factors;    fermented feed;    high-throughput sequencing;    Procambarus clarkii;   

DOI  :  10.3390/microorganisms8091376

来源: DOAJ

【 摘 要 】

Red swamp crayfish (Procambarus clarkii) breeding is an important economic mainstay in Hubei province, China. However, information on the gut microbiota of the red swamp crayfish is limited. To address this issue, the effect of developmental stage, diet (fermented or non-fermented feed), and geographical location on the gut microbiota composition in the crayfish was studied via high-throughput 16S rRNA gene sequencing. The results revealed that the dominant phyla in the gut of the crayfish were Proteobacteria, Bacteroidetes,Firmicutes, Tenericutes, and RsaHF231. The alpha diversity showed a declining trend during development, and a highly comparable gut microbiota clustering was identified in a development-dependent manner. The results also revealed that development, followed by diet, is a better key driver for crayfish gut microbiota patterns than geographical location. Notably, the relative abundance of Bacteroidetes was significantly higher in the gut of the crayfish fed with fermented feed than those fed with non-fermented feed, suggesting the fermented feed can be important for the functions (e.g., polysaccharide degradation) of the gut microbiota. In summary, our results revealed the factors shaping gut microbiota of the crayfish and the importance of the fermented feed in crayfish breeding.

【 授权许可】

Unknown