Temporal proteomic profiling reveals functional pathways in vaccinia virus-induced cell migration

Frontiers in Microbiology

Microbiology

Wei Liu¹  Xue-Zhu Chen¹  Liang Qiao¹  Yan-Jun Liu¹  Baohong Liu¹  Jiayin Lu¹  Yiwen Wang¹  Tianlei Ying² 

[1] Department of Chemistry, Shanghai Stomatological Hospital, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China;MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Fudan University, Shanghai, China;

关键词: temporal proteomics;    vaccinia virus;    cell migration;    host-virus interactions;    regulation of actin cytoskeleton;   

DOI  :  10.3389/fmicb.2023.1185960

 received in 2023-03-17, accepted in 2023-04-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionViral diseases have always been intricate and persistent issues throughout the world and there is a lack of holistic discoveries regarding the molecular dysregulations of virus-host interactions. The temporal proteomics strategy can identify various differentially expressed proteins and offer collaborated interaction networks under pathological conditions.MethodHerein, temporal proteomics at various hours post infection of Vero cells were launched to uncover molecular alternations during vaccinia virus (VACV)-induced cell migration. Different stages of infection were included to differentiate gene ontologies and critical pathways at specific time points of infection via bioinformatics.ResultsBioinformatic results showed functional and distinct ontologies and pathways at different stages of virus infection. The enrichment of interaction networks and pathways verified the significances of the regulation of actin cytoskeleton and lamellipodia during VACV-induced fast cell motility.DiscussionThe current results offer a systematic proteomic profiling of molecular dysregulations at different stages of VACV infection and potential biomedical targets for treating viral diseases.

【 授权许可】

Unknown   
Copyright © 2023 Lu, Liu, Chen, Wang, Ying, Qiao, Liu and Liu.