Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120

Frontiers in Microbiology

Microbiology

Sadhana Yadav¹  Rajesh Prajapati¹  Vigya Kesari¹  Nidhi Singh¹  Rupanshee Srivastava¹  Tripti Kanda¹  Neelam Atri²  Shivam Yadav³ 

[1] Department of Botany, Institute of Sciences, Banaras Hindu University, Varanasi, India;Department of Botany, Mahila Mahavidyalaya (M.M.V.), Banaras Hindu University, Varanasi, India;Department of Botany, Thakur Prasad Singh (T.P.S.) College, Patna, Bihar, India;

关键词: cyanobacteria;    Anabaena;    salinity;    heat shock;    pretreatments;    stress;   

DOI  :  10.3389/fmicb.2023.1061927

 received in 2022-10-05, accepted in 2023-01-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

This study was undertaken to bridge the knowledge gap pertaining to cyanobacteria’s response to pretreatment. The result elucidates the synergistic effect of pretreatment toxicity in cyanobacterium Anabaena PCC7120 on morphological and biochemical attributes. Chemical (salt) and physical (heat) stress-pretreated cells exhibited significant and reproducible changes in terms of growth pattern, morphology, pigments, lipid peroxidation, and antioxidant activity. Salinity pretreatment showed more than a five-fold decrease in the phycocyanin content but a six-fold and five-fold increase in carotenoid, lipid peroxidation (MDA content), and antioxidant activity (SOD and CAT) at 1 h and on 3rd day of treatment, respectively, giving the impression of stress-induced free radicals that are scavenged by antioxidants when compared to heat shock pretreatment. Furthermore, quantitative analysis of transcript (qRT-PCR) for FeSOD and MnSOD displayed a 3.6- and 1.8-fold increase in salt-pretreated (S-H) samples. The upregulation of transcript corresponding to salt pretreatment suggests a toxic role of salinity in synergizing heat shock. However, heat pretreatment suggests a protective role in mitigating salt toxicity. It could be inferred that pretreatment enhances the deleterious effect. However, it further showed that salinity (chemical stress) augments the damaging effect of heat shock (physical stress) more profoundly than physical stress on chemical stress possibly by modulating redox balance via activation of antioxidant responses. Our study reveals that upon pretreatment of heat, the negative effect of salt can be mitigated in filamentous cyanobacteria, thus providing a foundation for improved cyanobacterial tolerance to salt stress.

【 授权许可】

Unknown   
Copyright © 2023 Srivastava, Kanda, Yadav, Singh, Yadav, Prajapati, Kesari and Atri.