Complementarity determining regions in SARS-CoV-2 hybrid immunity

Frontiers in Immunology

Immunology

Nico Callewaert¹  Lina Ancheva²  Lise Boon²  Doreen Dillaerts²  Tom Dehaemers²  Jonas De Leeuw²  Maaike Cockx²  Glynis Frans³  Jan Van Elslande³  Xavier Bossuyt⁴  Pieter Vermeersch⁵  Bas Calcoen⁶  Nick Geukens⁷  Wim Maes⁷  Sebastien Christian Carpentier⁸  Kusay Arat⁸  Rita Derua⁹ 

[1] Clinical Laboratory, AZ Groeninge Hospital, Kortrijk, Belgium;Clinical and Diagnostic Immunology, Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium;Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium;Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium;Clinical and Diagnostic Immunology, Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium;Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium;Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium;Laboratory for Thrombosis Research, KU Leuven Kulak Kortrijk, Kortrijk, Belgium;PharmAbs: The KU Leuven Antibody Center, KU Leuven, Leuven, Belgium;SyBioMa, KU Leuven, Leuven, Belgium;SyBioMa, KU Leuven, Leuven, Belgium;Department of Molecular and Cellular Medicine, Laboratory of Protein Phosphorylation and Proteomics, KU Leuven, Leuven, Belgium;

关键词: SARS-CoV-2;    COVID-19;    hybrid immunity;    complementary determining region;    vaccination;   

DOI  :  10.3389/fimmu.2023.1050037

 received in 2022-09-21, accepted in 2023-02-02,  发布年份 2023

来源: Frontiers

【 摘 要 】

Pre-vaccination SARS-CoV-2 infection can boost protection elicited by COVID-19 vaccination and post-vaccination breakthrough SARS-CoV-2 infection can boost existing immunity conferred by COVID-19 vaccination. Such ‘hybrid immunity’ is effective against SARS-CoV-2 variants. In order to understand ‘hybrid immunity’ at the molecular level we studied the complementarity determining regions (CDR) of anti-RBD (receptor binding domain) antibodies isolated from individuals with ‘hybrid immunity’ as well as from ‘naive’ (not SARS-CoV-2 infected) vaccinated individuals. CDR analysis was done by liquid chromatography/mass spectrometry-mass spectrometry. Principal component analysis and partial least square differential analysis showed that COVID-19 vaccinated people share CDR profiles and that pre-vaccination SARS-CoV-2 infection or breakthrough infection further shape the CDR profile, with a CDR profile in hybrid immunity that clustered away from the CDR profile in vaccinated people without infection. Thus, our results show a CDR profile in hybrid immunity that is distinct from the vaccination-induced CDR profile.

【 授权许可】

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Copyright © 2023 Frans, Dillaerts, Dehaemers, Van Elslande, De Leeuw, Boon, Maes, Callewaert, Calcoen, Ancheva, Cockx, Geukens, Arat, Derua, Vermeersch, Carpentier and Bossuyt