Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index

Frontiers in Microbiology

Microbiology

Youngjae Yu¹  Junhee Ryu²  Jihyang Kweon³  Jaehyun Jung⁴  Suyoung Choi⁵  Wonjung Song⁵ 

[1] Department of Chemical and Environmental Engineering, University of Arizona, Tucson, AZ, United States;Department of Civil and Environmental Engineering, Konkuk University, Seoul, Republic of Korea;Department of Environmental Engineering Konkuk University, Seoul, Republic of Korea;HANSU Technical Service Ltd, Sungnam-si, Gyeonggi-do, Republic of Korea;The Academy of Applied Science and Technology, Konkuk University, Seoul, Republic of Korea;

关键词: membrane bioreactor;    cis;    diffusible signal factor (DSF);    quorum sensing;    biofilm;    extracellular polymeric substances (EPS);   

DOI  :  10.3389/fmicb.2023.1211761

 received in 2023-04-25, accepted in 2023-07-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionBiofilm occurs ubiquitously in water system. Excessive biofilm formation deteriorates severely system performance in several water and wastewater treatment processes. Quorum sensing systems were controlled in this study with a signal compound cis-2-Decenoic acid (CDA) to regulate various functions of microbial communities, including motility, enzyme production, and extracellular polymeric substance (EPS) production in biofilm.MethodsThe addition of CDA to six strains extracted from membrane bioreactor sludge and the Pseudomonas aeruginosa PAO1 strain was examined for modulating biofilm development by regulating DSF expression.Results and discussionAs the CDA doses increased, optical density of the biofilm dispersion assay increased, and the decrease in EPS of the biofilm was obvious on membrane surfaces. The three-dimensional visual images and quantitative analyses of biofilm formation with CDA proved thinner, less massive, and more dispersive than those without; to evaluate its dispersive intensity, a dispersion index was proposed. This could compare the dispersive effects of CDA dosing to other biofilms or efficiencies of biofouling control practices such as backwashing or new cleaning methods.

【 授权许可】

Unknown   
Copyright © 2023 Song, Ryu, Jung, Yu, Choi and Kweon.