Stability and bandwidth investigation of alternative structures for nanopore sensors

Nanopore;Bandwidth;Biopore;Alumina;Aluminum Oxide (Al2O3);Silicon Nitride (Si3N4);electrolyte;Deoxyribonucleic Acid (DNA);cis;trans

Yemenicioglu, Sukru ; Bashir ; Rashid

关键词: Nanopore;    Bandwidth;    Biopore;    Alumina;    Aluminum Oxide (Al2O3);    Silicon Nitride (Si3N4);    electrolyte;    Deoxyribonucleic Acid (DNA);    cis;    trans;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/16989/Yemenicioglu_Sukru.pdf?sequence=3&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

The genetic information carriers, DNA molecules can be thought of as theblueprints of living organisms. This crucial functionality of the DNA mole-cules may explain the drive and momentum for DNA sequencing research.The commonly used parallel sequencing methods require extensive samplepreparation, long processing times and expensive chemical reagents. In or-der to realize the goal of $1000 genome sequencing, many alternative meth-ods are proposed. One of the most promising technologies among these isnanopore sequencing. Nanopore sequencing involves the threading of a DNAmolecule between two electrolytic reservoirs through a nanometer-sized poreon a synthetic or an organic platform by means of electrophoresis and/or mag-netism. During the threading of DNA molecules, various electrical aspectsof the bases are investigated. The minimal label-free sample preparation,possibility of parallelizability and high throughput are the factors that makethis method a very promising solution for low-cost, robust and fast DNA se-quencing. In the nanopore sequencingeld, the synthetic platforms have theadvantage of durability and mass production value due to the existing sili-con device fabrication technologies. This thesis work focuses on the stabilityand bandwidth investigation of alternative structures for nanopore sensing.Membranes with various thicknesses of Al2O3, Si3N4 and SiO2 stack con gu-rations were fabricated. The fabricated membranes were analyzed and drilledthrough by focused e-beam sputtering in TEM. The membranes were testedin 0.1 M and 1 M KCl solutions for IV characteristics, noise level and ACresponse. The membranes with desirable noise and IV characteristics werefurther tested for DNA sensing purposes. The membranes featuring Al2O3insulating layer con gurations yielded low noise, high bandwidth and lim-ited durability in KCl solutions. The low yield in DNA sensing in 1 M KClsolutions using these architectures forms the background and motivation fornext generation structures for DNA sensing.