Transcriptional and proteomic analysis of the innate immune response to microbial stimuli in a model invertebrate chordate

Frontiers in Immunology

Immunology

Antonietta Spagnuolo¹  Assunta Liberti¹  Rita Marino¹  Carla Pollastro¹  Paolo Sordino²  Gabriella Pinto³  Anna Illiano³  Angela Amoresano³ 

[1] Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dohrn, Naples, Italy;Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dohrn, Sicily Marine Centre, Messina, Italy;Department of Chemical Sciences, University of Naples Federico II, Naples, Italy;Istituto Nazionale Biostrutture e Biosistemi-Consorzio Interuniversitario, Rome, Italy;

关键词: inflammatory response;    innate immunity;    Ciona robusta;    marine invertebrates;    microbial stimuli;    transcriptional analysis;    proteomic analysis;    protein-protein interactome;   

DOI  :  10.3389/fimmu.2023.1217077

 received in 2023-05-04, accepted in 2023-07-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Inflammatory response triggered by innate immunity can act to protect against microorganisms that behave as pathogens, with the aim to restore the homeostatic state between host and beneficial microbes. As a filter-feeder organism, the ascidian Ciona robusta is continuously exposed to external microbes that may be harmful under some conditions. In this work, we used transcriptional and proteomic approaches to investigate the inflammatory response induced by stimuli of bacterial (lipopolysaccharide -LPS- and diacylated lipopeptide - Pam2CSK4) and fungal (zymosan) origin, in Ciona juveniles at stage 4 of metamorphosis. We focused on receptors, co-interactors, transcription factors and cytokines belonging to the TLR and Dectin-1 pathways and on immune factors identified by homology approach (i.e. immunoglobulin (Ig) or C-type lectin domain containing molecules). While LPS did not induce a significant response in juvenile ascidians, Pam2CSK4 and zymosan exposure triggered the activation of specific inflammatory mechanisms. In particular, Pam2CSK4-induced inflammation was characterized by modulation of TLR and Dectin-1 pathway molecules, including receptors, transcription factors, and cytokines, while immune response to zymosan primarily involved C-type lectin receptors, co-interactors, Ig-containing molecules, and cytokines. A targeted proteomic analysis enabled to confirm transcriptional data, also highlighting a temporal delay between transcriptional induction and protein level changes. Finally, a protein-protein interaction network of Ciona immune molecules was rendered to provide a wide visualization and analysis platform of innate immunity. The in vivo inflammatory model described here reveals interconnections of innate immune pathways in specific responses to selected microbial stimuli. It also represents the starting point for studying ontogeny and regulation of inflammatory disorders in different physiological conditions.

【 授权许可】

Unknown   
Copyright © 2023 Liberti, Pollastro, Pinto, Illiano, Marino, Amoresano, Spagnuolo and Sordino