Rapid visual characterization of alkaloid changes in traditional processing of Tibetan medicine Aconitum pendulum by high-performance thin-layer chromatography coupled with desorption electrospray ionization mass spectrometry imaging

Frontiers in Pharmacology

Pharmacology

Zhaoqing Pei¹  Congying Li²  Yue Liu²  Qingxiu He²  Zige Feng²  Xiaoyan Tan²  Ce Tang³  Yi Zhang³ 

[1] Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China;School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China;School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China;Meishan Hosptial of Chengdu University of Traditional Chinese Medicine, Meishan, China;

关键词: DESI-MSI;    HPTLC;    radix aconiti;    processing;    alkaloids;   

DOI  :  10.3389/fphar.2023.1104473

 received in 2022-11-21, accepted in 2023-04-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Radix Aconiti, also known as Tie-bang-chui (TBC), Pang-a-na-bao, and Bang-na, is a typical aconitum Tibetan medicine and a perennial herb of the genus Aconitum pendulum Busch. and A. flavum Hand. -Mazz. dry roots. It has high toxicity and remarkable efficacy; as such, it is a typical “highly toxic and effective” drug that needs be processed and used. Processing methods of this Tibetan medicine include non-heating of highland barley wine (HBW) and fructus chebulae soup (FCS). This work aimed to understand differences in chemical composition between non-heat processed products and raw TBC. In this study, high-performance thin-layer chromatography (HPTLC) and desorption electrospray ionization mass spectrometry imaging (DESI-MSI) were used to analyze the chemical composition of TBC processed by FCS (F-TBC) and HBW (H-TBC). The MRM mode of HPLC-QqQ-MS/MS was selected to determine the changes of several representative alkaloids to comparison with the former results. A total of 52 chemical constituents were identified in raw and processed products, and the chemical composition of F-TBC and H-TBC changed slightly compared with that of raw TBC. The processing mechanism of H-TBC was also different from that of F-TBC, which might be related to the large amount of acidic tannins in FCS. It was found that the content of all six alkaloids decreased after processing by FCS, and all five alkaloids decreased except aconitine increased after processing by HBW. The combination of HPTLC and DESI-MSI could be an effective method for rapid identification of chemical components and changing rules in ethnic medicine. The wide application of this technology provides not only an alternative method for the traditional separation and identification of secondary metabolism but also a reference for research on the processing mechanism and quality control of ethnic medicine.

【 授权许可】

Unknown   
Copyright © 2023 Tan, He, Pei, Liu, Feng, Li, Tang and Zhang.