Genetic characteristic of coexisting of mcr-1 and blaNDM-5 in Escherichia coli isolates from lesion-bearing animal organs

Frontiers in Microbiology

Microbiology

Zengyuan Liu¹  Xujing Geng²  Yan Li²  Yungai Xiang²  Guo Yu²  Yuxia Song²  Liying Ma²  Li Tan²  Junqing Guo³  Pengju Chen⁴ 

[1] College of Pharmacy, Shenzhen Technology University, Shenzhen, China;Department of Reproductive Medicine, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China;Henan Institute of Modern Chinese Veterinary Medicine, Zhengzhou, Henan, China;Henan Institute of Modern Chinese Veterinary Medicine, Zhengzhou, Henan, China;Shandong Xindehui Biotechnology Company Ltd., Yuncheng, Shandong, China;

关键词: Escherichia coli;    mcr-1;    bla;    lesions-bearing;    chromosome;   

DOI  :  10.3389/fmicb.2023.1116413

 received in 2022-12-05, accepted in 2023-02-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

The coexistence of mcr-1 and blaNDM-5 in the plasmid of Escherichia coli has been widely reported and such strains have been mainly isolated from animal and human feces. However, few reports have focused on the genetic diversity of mcr-1-carrying chromosomes and blaNDM-5-carrying plasmids in E. coli isolates from lesion-bearing animal organs. This study investigated the genetic characteristics of chromosome-mediated mcr-1 and plasmid-mediated blaNDM-5 in E. coli isolated from lesion-bearing animal organs. Nine mcr-1- and blaNDM-5-positive E. coli strains (MNPECs) showed extensive drug resistance (XDR). The predominant clonal complexes (CC) mainly belonged to CC156, CC10, and CC165 from the 56 MNEPCs (including nine strains in this study) retrieved from the literature. These strains were widely distributed in China, and originated from pig fecal samples, human stool/urine samples as well as intestinal contents of chicken. Two transconjugants harboring blaNDM-5 gene were also successfully obtained from two donors (J-8 and N-14) and this transfer increased the MIC for meropenem by 256 times. However, conjugative transfer of mcr-1 gene failed. Both J-8 and N-14 strains contained point mutations associated with quinolone resistance and more than three types of AMR genes, including the mcr-1 gene on the chromosome and the blaNDM-5 gene on the IncX3-type plasmid. The genetic structure of mcr-1 located on the chromosome was an intact Tn6330, and blaNDM-5-carrying IncX3-type plasmid was ISAb125-IS5-blaNDM-5-bleO-trpF-tat-cutA-IS26 gene cassette. Moreover, differences between chromosomes included additional partial sequence of phage integrated into host genome and the different genes associated with O-antigen synthesis.

【 授权许可】

Unknown   
Copyright © 2023 Xiang, Liu, Yu, Song, Li, Geng, Ma, Guo, Tan and Chen.