Voluntary exercise does not increase gastrointestinal motility but increases spatial memory, intestinal eNOS, Akt levels, and Bifidobacteria abundance in the microbiome

Frontiers in Physiology

Physiology

Jonguk Park¹  Kinga Molnár²  Lajos László²  Balazs Kutasi³  Kumpei Tanisawa⁴  Yaodong Gu⁵  Ricardo A. Pinho⁶  Soroosh Mozaffaritabar⁷  Attila Kolonics⁷  Lei Zhou⁷  Peter Bakonyi⁷  Dora Aczel⁷  Zsolt Radak⁸ 

[1] Artificial Intelligence Center for Health and Biomedical Research, Osaka, Japan;Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University of Sciences, Budapest, Hungary;Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary;Faculty of Sport Sciences, Waseda University, Tokorozawa, Japan;Faculty of Sports Science, Ningbo University, Ningbo, China;Laboratory of Exercise Biochemistry in Health, Graduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica Do Paraná, Curitiba, Brazil;Research Institute of Sport Science, Hungarian University of Sport Science, Budapest, Hungary;Research Institute of Sport Science, Hungarian University of Sport Science, Budapest, Hungary;Faculty of Sport Sciences, Waseda University, Tokorozawa, Japan;

关键词: exercise;    intestine;    microbiome;    motility;    caveolae;   

DOI  :  10.3389/fphys.2023.1173636

 received in 2023-02-24, accepted in 2023-07-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

The interaction between the gut and brain is a great puzzle since it is mediated by very complex mechanisms. Therefore, the possible interactions of the brain–exercise–intestine–microbiome axis were investigated in a control (C, N = 6) and voluntarily exercised (VE, N = 8) middle-aged rats. The endurance capacity was assessed by VO2max on the treadmill, spatial memory by the Morris maze test, gastrointestinal motility by EMG, the microbiome by 16S RNA gene amplicon sequencing, caveolae by electron microscopy, and biochemical assays were used to measure protein levels and production of reactive oxygen species (ROS). Eight weeks of voluntary running increased VO2max, and spatial memory was assessed by the Morris maze test but did not significantly change the motility of the gastrointestinal tract or production of ROS in the intestine. The protein kinase B (Akt) and endothelial nitric oxide synthase (eNOS) protein levels significantly increased in the intestine, while peroxisome proliferator–activated receptor gamma coactivator 1 alpha (PGC-1α), mitochondrial transcription factor A (TFAM), nuclear respiratory factor 1 (NFR1), SIRT1, SIRT3, nicotinamide phosphoribosyl transferase (NAMPT), and nuclear factor κB (NF-κB) did not change. On the other hand, voluntary exercise increased the number of caveolae in the smooth muscles of the intestine and relative abundance of Bifidobacteria in the microbiome, which correlated with the Akt levels in the intestine. Voluntary exercise has systemic effects and the relationship between intestinal Akt and the microbiome of the gastrointestinal tract could be an important adaptive response.

【 授权许可】

Unknown   
Copyright © 2023 Bakonyi, Kolonics, Aczel, Zhou, Mozaffaritabar, Molnár, László, Kutasi, Tanisawa, Park, Gu, Pinho and Radak.