Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection

Frontiers in Microbiology

Microbiology

Yongqing Li¹  Yanyu Guo²  Lilin Zhang²  Huixia Zhang²  Ruyu Xie²  Shujun Li²  Zexing Li²  Han Zhang²  Changyan Li²  Xinyi Zhang²  Daqing Cui²  Jinhai Huang²  Liu Yang² 

[1] Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing, China;School of Life Sciences, Tianjin University, Tianjin, China;

关键词: avian influenza virus;    Saccharomyces cerevisiae;    innate immunity;    vaccine;    chicken;   

DOI  :  10.3389/fmicb.2023.1153922

 received in 2023-01-30, accepted in 2023-04-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionProphylactic vaccination is regarded as the most effective means to control avian flu infection. Currently, there is a need for a universal vaccine that provides broad and long-lasting protection against influenza virus. Meanwhile, although yeast-based vaccines have been used in clinic, studies are still required to further understand the molecular mechanism of yeast-based vaccines under physiological conditions.MethodsWe generated a yeast-based vaccine against influenza hemagglutinin (HA) of H5, H7 and H9 using surface displaying technology and evaluated the protective efficacy of chickens after exposure to H9N2 influenza virus.ResultsOral yeast vaccine provided less clinical syndrome, reduced viral loading and alleviated airway damage significantly. Compared to the commercial inactivated vaccine, yeast vaccine stimulated the activation of splenic NK and APCs cells and boosted TLR7-IRF7-IFN signaling in spleen. Meanwhile, γδ T cells in the bursa of Fabricius were activated and the innate lymphoid cells (ILCs) in the bursa of Fabricius promoted the CILPs to differentiate to ILC3 cells in oral yeast birds. Moreover, the reshaped gut microbiota and a suppressed Th17-IL17-mediated inflammation in intestine was observed in oral yeast chickens, which might facilitate the recovery of intestinal mucosal immunity upon virus infection. Collectively, our findings suggest that oral yeast based multivalent bird flu vaccines provide an attractive strategy to update host defense function via reshapes of multi-systemic immune homeostasis.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Li, Zhang, Guo, Zhang, Zhang, Yang, Li, Li, Cui, Xie, Li and Huang.