Axenic in vitro cultivation and genome diploidization of the moss Vesicularia montagnei for horticulture utilization

Frontiers in Plant Science

Plant Science

Qing Li¹  Congfa Wen²  Hong Yang³  Hongyu Li³  Zhanwu Xu³  Zexi Chen³  Li Liu³  Yong Hu³  Liu Duan³ 

[1] Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China;University of Chinese Academy of Sciences, Beijing, China;Lishui Runsheng Moss Technology Co., Ltd. Green Valley Information Industrial Park, Lishui, Zhejiang, China;State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, China;

关键词: diploidization;    moss;    cultivation;    horticulture;    Vesicularia montagnei;   

DOI  :  10.3389/fpls.2023.1137214

 received in 2023-01-04, accepted in 2023-03-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

Mosses are widely used in the establishment of greenery. However, little research has been conducted to choose a suitable species or improve their performance for this application. In our study, we examined Vesicularia montagnei (V. montagnei), a robust moss that is widely distributed in temperate, subtropical, and tropical Asia with varying environmental conditions. Axenic cultivation system of V. montagnei was developed on modified BCD medium, which enabled its propagation and multiplication in vitro. In this axenic cultivation environment, several diploid V. montagnei lines with enhancement of rhizoid system were generated through artificial induction of diploidization. Transcriptomic analysis revealed that several genes responsible for jasmonic acid (JA) biosynthesis and signaling showed significant higher expression levels in the diploid lines compared to the wild type. These results are consistent with the increasement of JA content in the diploid lines. Our establishment of the axenic cultivation method may provide useful information for further study of other Vesicularia species. The diploid V. montagnei lines with improved rhizoid system may hold promising potential for greenery applications. Additionally, our study sheds light on the biosynthesis and functions of JA in the early landed plants.

【 授权许可】

Unknown   
Copyright © 2023 Hu, Li, Chen, Xu, Li, Wen, Duan, Yang and Liu