Whole‐Genome Sequence of Synthesized Allopolyploids in Cucumis Reveals Insights into the Genome Evolution of Allopolyploidization

Advanced Science

Sanwen Huang¹  Haiyan Yu²  Xinchao Cheng²  Junguo Zhou³  Qinsheng Cai⁴  Carl‐Otto Ottosen⁵  Yuling Bai⁶  Shiro Isshiki⁷  Changmian Ji⁸  Molly Jahn⁹  Qunfeng Lou^1,10  Jinfeng Chen^1,10  Panqiao Wang^1,10  Xiaodong Qin^1,10  Yufei Zhai^1,10  Xiaqing Yu^1,10  Zaobing Zhu^1,10  Qinzheng Zhao^1,10  Ji Li^1,10  Chunyan Cheng^1,10  Jeff J. Doyle^1,11 

[1] Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518124 China;Biomarker Technologies Beijing 101300 China;College of Horticulture and Landscape Henan Institute of Science and Technology Xinxiang 453000 China;College of Life Science Nanjing Agricultural University Nanjing 210095 China;Department of Food Science Aarhus University Aarhus 8200 Denmark;Department of Plant Sciences Wageningen University and Research Wageningen 6700 AJ Netherlands;Faculty of Agriculture Saga University Saga 840‐8502 Japan;Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off‐Season Reproduction Regions Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China;Jahn Research Group USDA/FPL Madison WI 53726 USA;National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China;Section of Plant Breeding and Genetics School of Integrated Plant Sciences Cornell University Ithaca NY 14853 USA;

关键词: allopolyploidy;    Cucumis;    diploidization;    evolution;    genomes;   

DOI  :  10.1002/advs.202004222

来源: DOAJ

【 摘 要 】

Abstract The importance of allopolyploidy in plant evolution has been widely recognized. The genetic changes triggered by allopolyploidy, however, are not yet fully understood due to inconsistent phenomena reported across diverse species. The construction of synthetic polyploids offers a controlled approach to systematically reveal genomic changes that occur during the process of polyploidy. This study reports the first fully sequenced synthetic allopolyploid constructed from a cross between Cucumis sativus and C. hystrix, with high‐quality assembly. The two subgenomes are confidently partitioned and the C. sativus‐originated subgenome predominates over the C. hystrix‐originated subgenome, retaining more sequences and showing higher homeologous gene expression. Most of the genomic changes emerge immediately after interspecific hybridization. Analysis of a series of genome sequences from several generations (S0, S4–S13) of C. ×hytivus confirms that genomic changes occurred in the very first generations, subsequently slowing down as the process of diploidization is initiated. The duplicated genome of the allopolyploid with double genes from both parents broadens the genetic base of C. ×hytivus, resulting in enhanced phenotypic plasticity. This study provides novel insights into plant polyploid genome evolution and demonstrates a promising strategy for the development of a wide array of novel plant species and varieties through artificial polyploidization.

【 授权许可】

Unknown