Comparison of the 3D-microstructure of human alveolar and fibula bone in microvascular autologous bone transplantation: a synchrotron radiation μ-CT study

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Susanne Nahles¹  Carsten Rendenbach¹  Max Heiland¹  Jonas Wüster¹  Saskia Preissner¹  Tobias Fretwurst²  Rene Rothweiler²  Katja Nelson²  Christian Gross²  Elodie Boller³  Andrew King⁴  Shima Bakhtiyari⁵  Emely Bortel⁵  Javier Gerber⁵  Bernhard Hesse⁶ 

[1] Department of Oral and Maxillofacial Surgery, Berlin Institute of Health, Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany;Department of Oral- and Craniomaxillofacial Surgery, Faculty of Medicine, Medical Center, University of Freiburg, Freiburg, Germany;European Synchrotron Radiation Facility, Grenoble, France;Synchrotron Soleil, Saint-Aubin, France;Xploraytion GmbH, Berlin, Germany;Xploraytion GmbH, Berlin, Germany;European Synchrotron Radiation Facility, Grenoble, France;

关键词: osteocyte lacunae;    bone microarchitecture;    SR micro-CT;    synchrotron;    free fibula flap;    bone vessel orientation;    scaffold;   

DOI  :  10.3389/fbioe.2023.1169385

 received in 2023-02-19, accepted in 2023-08-01,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Autologous bone transplantation is successfully used in reconstructive surgery of large/critical-sized bone defects, whereby the microvascular free fibula flap is still regarded as the gold standard for the reconstruction of such defects in the head and neck region. Here, we report the morphological and lacunar properties of patient-paired bone samples from eight patients from the jaw (AB; recipient site) and the fibula (FB; donor site) on the micron length-scale using Synchrotron µ-CT. Insights into differences and similarities between these bone structures could offer a better understanding of the underlying mechanism for successful surgical outcomes and might clear the path for optimized, nature-inspired bone scaffold designs.Methods: Spatial vessel-pore arrangements, bone morphology, fluid-simulation derived permeability tensor, osteocyte lacunar density, and lacunar morphology are compared.Results: The orientation of the vessel system indicates a homogenous vessel orientation for AB and FB. The average mineral distance (50%) to the closest vessel boundary is higher in AB than in FB (the mean is 96 μm for AB vs. 76 μm for FB; p = 0.021). Average osteocyte lacunar density is found to be higher in AB than in FB (mean 22,874 mm3 vs. 19,376 mm3 for FB; p = 0.038), which might compensate for the high distance from the mineral to the nearest vessel. No significant differences in lacunar volume are found between paired AB and FB.Discussion: A comparable vessel network and similar distribution of vessel porosity between AB and FB may allow the FB graft to exhibit a high regeneration potential when connected to AB, and this might correlate with a high osteoinductive and osteoconductive potential of FB when connected to AB. Since widely used and potent synthetic bone grafts exist, new insight into the bone structure of well-established autologous bone grafts, such as the free fibula flap, could help to improve the performance of such materials and therefore the design of 3D scaffolds.

【 授权许可】

Unknown   
Copyright © 2023 Wüster, Hesse, Rothweiler, Bortel, Gross, Bakhtiyari, King, Boller, Gerber, Rendenbach, Fretwurst, Preissner, Heiland, Nelson and Nahles.