EBR and JA regulate aroma substance biosynthesis in ‘Ruidu Hongyu’ grapevine berries by transcriptome and metabolite combined analysis

Frontiers in Plant Science

Plant Science

Hafiz Umer Javed¹  Lei Wang²  Caixi Zhang²  Jiajia Li²  Yi Quan²  Zishu Wu²  Shiping Wang²  Chao Ma²  Songtao Jiu²  Jiayu Han³  Ying Zhang³ 

[1] College of Chemistry and Chemical Engineering, Zhongkai University of Agricultural Engineering, Guangzhou, China;Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China;Grape and Wine Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China;

关键词: grapevine;    EBR;    JA;    HS-SPME/GC−MS;    aroma;    ‘Ruidu Hongyu’;    transcriptome sequencing;   

DOI  :  10.3389/fpls.2023.1185049

 received in 2023-03-13, accepted in 2023-05-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

Volatile compounds including terpenes, aldehyde, phenol, and alcohol are significantly contributed floral and fruity aromas to the Muscat variety. ‘Ruidu Hongyu’ grapevine is one of the newly developed grape varieties, and cultivation of this variety has been extended across China due to unique quality traits and taste. In this study, HS-SPME/GC−MS and transcriptome sequencing analysis were performed to evaluate the impact of exogenous 2,4-epibrassinolide (EBR), jasmonic acid (JA), and their signaling inhibitors brassinazole (Brz)/sodium diethyldithiocarbamate (DIECA) on the biosynthesis of aroma substances in ‘Ruidu Hongyu’ grapevine. According to the results, exogenous BR and JA promoted the accumulation of various aroma substances, including hexenal, 2-hexenal, nerol oxide, vanillin, hotrienol, terpineol, neral, nerol, geraniol, and geranic acid. After EBR and JA treatments, most of the genes responsible for terpene, aldehyde, and alcohol biosynthesis expressed at a higher level than the CK group. Relatively, EBR treatment could not only promote endogenous BR biosynthesis and metabolism but also elevate BR signaling transduction. JA treatment contributed to endogenous JA and MeJA accumulation, as well. Through transcriptome sequencing, a total of 3043, 903, 1470, and 607 DEGs were identified in JA vs. JD, JA vs. CK, BR vs. CK, and BR vs. Brz, respectively. There were more DEGs under both EBR and JA treatments at late fruit ripening stages. The findings of this study increase our understanding regarding aroma substances biosynthesis and endogenous BR/JA metabolism in response to exogenous EBR and JA signals.

【 授权许可】

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Copyright © 2023 Li, Quan, Wu, Han, Zhang, Javed, Ma, Jiu, Zhang, Wang and Wang