Frontiers in Immunology | |
Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research | |
Immunology | |
Wei Li1  Xiaoying Wei1  Yan Qin1  Yan Zhang2  Hanyi Ruan2  Yujie Zhang2  Tianyu Qin2  | |
[1] Department of Health Management, The People’s Hospital of Guangxi Zhuang Autonomous Region and Research Center of Health Management, Guangxi Academy of Medical Sciences, Nanning, China;Department of Oncology, Guangxi Medical University Cancer Hospital, Nanning, China; | |
关键词: IFN-γ; tumor microenvironment; immunotherapy; pan-cancer; single-cell transcriptome sequencing; | |
DOI : 10.3389/fimmu.2023.1202150 | |
received in 2023-04-07, accepted in 2023-07-17, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
BackgroundInterferon-gamma (IFN-γ), commonly referred to as type II interferon, is a crucial cytokine that coordinates the tumor immune process and has received considerable attention in tumor immunotherapy research. Previous studies have discussed the role and mechanisms associated with IFN-γ in specific tumors or diseases, but the relevant role of IFN-γ in pan-cancer remains uncertain.MethodsTCGA and GTEx RNA expression data and clinical data were downloaded. Additionally, we analyzed the role of IFN-γ on tumors by using a bioinformatic approach, which included the analysis of the correlation between IFN-γ in different tumors and expression, prognosis, functional status, TMB, MSI, immune cell infiltration, and TIDE. We also developed a PPI network for topological analysis of the network, identifying hub genes as those having a degree greater than IFN-γ levels.ResultIFN-γ was differentially expressed and predicted different survival statuses in a majority of tumor types in TCGA. Additionally, IFN-γ expression was strongly linked to factors like infiltration of T cells, immune checkpoints, immune-activating genes, immunosuppressive genes, chemokines, and chemokine receptors, as well as tumor purity, functional statuses, and prognostic value. Also, prognosis, CNV, and treatment response were all substantially correlated with IFN-γ-related gene expression. Particularly, the IFN-γ-related gene STAT1 exhibited the greatest percentage of SNVs and the largest percentage of SNPs in UCEC. Elevated expression levels of IFN-γ-related genes were found in a wide variety of tumor types, and this was shown to be positively linked to drug sensitivity for 20 different types of drugs.ConclusionIFN-γ is a good indicator of response to tumor immunotherapy and is likely to limit tumor progression, offering a novel approach for immunotherapy’s future development.
【 授权许可】
Unknown
Copyright © 2023 Wei, Ruan, Zhang, Qin, Zhang, Qin and Li
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