Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children

Frontiers in Immunology

Immunology

Fang Liu¹  Anxia Jiao¹  Xiaohui Kang²  Baoying Zheng²  Shunying Zhao³  Jingang Gui⁴  Xi Chen⁴  Xiaolin Wang⁴  Wenjun Mou⁴  Hui Zhang⁴ 

[1] Department of Interventional Pulmonology, Beijing Children’s Hospital affiliated to the Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, Beijing, China;Department of Pulmonology, The Children’s Hospital Affiliated to the Capital Institute of Pediatrics, Beijing, China;Department of Respiratory Diseases, Beijing Children’s Hospital affiliated to the Capital Medical University, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, Beijing, China;Laboratory of Tumor Immunology, Beijing Pediatric Research Institute, Beijing Children’s Hospital affiliated to the Capital Medical University, National Center for Children’s Health, Beijing, China;

关键词: mycoplasma pneumoniae pneumonia (MPP);    children;    bronchoalveolar lavage fluid (BALF);    T cells;    proinflammatory milieu;    cell exhaustion;   

DOI  :  10.3389/fimmu.2021.760488

 received in 2021-08-19, accepted in 2021-12-20,  发布年份 2022

来源: Frontiers

【 摘 要 】

Severe mycoplasma pneumoniae pneumonia (MPP) in children presents with serious clinical complications. Without proper and prompt intervention, it could lead to deadly consequences. Dynamics of the inflammatory airway milieu and activation status of immune cells were believed to be the hallmark of the pathogenesis and progress of the disease. In this study, by employing the T-cell sorting and mRNA microarray, we were able to define the main feature of the chemokine/cytokine expression and the unique characteristics of T cells in the bronchoalveolar lavage fluid (BALF) from severe MPP patients at acute phase. Our study for the first time delineated the molecular changes in isolated BALF T cells in severe MPP children with respect to the cytokine/chemokine expression, cell activation, exhaustion, and apoptosis. By comparing the BALF aqueous expression of cytokines/chemokines with that in sorted T cells, our data give a preliminary clue capable of finishing out the possible cell source of the proinflammatory cytokines/chemokines from the BALF mixture. Meanwhile, our data provide a distinctively pellucid expression profile particularly belonging to the isolated BALF T cells demonstrating that in the inflammatory airway, overactivated T cells were exhausted and on the verge of apoptotic progress.

【 授权许可】

Unknown   
Copyright © 2022 Chen, Liu, Zheng, Kang, Wang, Mou, Zhang, Jiao, Zhao and Gui