Inhibitory insula-ACC projections modulate affective but not sensory aspects of neuropathic pain

Molecular Brain

Micro Report

Gabriel Oliveira de Melo¹  Camila Squarzoni Dale¹  Zizhen Zhang²  Gerald W. Zamponi²  Lina Chen²  Eder Gambeta²  Junting Huang²  Heloísa Alonso-Matielo³ 

[1] Department of Anatomy, Institute of Biomedical Sciences of University of São Paulo, Av. Prof. Lineu Prestes, 2415, ICB-III, Cidade Universitária, 05508-900, São Paulo, SP, Brazil;Department of Clinical Neurosciences, Alberta Children’s Hospital Research Institute and Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada;Department of Clinical Neurosciences, Alberta Children’s Hospital Research Institute and Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada;Department of Anatomy, Institute of Biomedical Sciences of University of São Paulo, Av. Prof. Lineu Prestes, 2415, ICB-III, Cidade Universitária, 05508-900, São Paulo, SP, Brazil;

关键词: Insular cortex;    Anterior cingulate cortex;    Neuropathic pain;    Neural projection;    Optogenetics;   

DOI  :  10.1186/s13041-023-01052-8

 received in 2023-07-13, accepted in 2023-08-14,  发布年份 2023

来源: Springer

【 摘 要 】

The insula and anterior cingulate cortex (ACC) are brain regions that undergo structural and functional reorganization in neuropathic pain states. Here, we aimed to study inhibitory parvalbumin positive (PV+) posterior insula (pIC) to posterior ACC (pACC) projections, and to evaluate the effects of direct optogenetic manipulation of such projections on mechanical nociception and spontaneous ongoing pain in mice with Spared Nerve Injury (SNI). CTB488 tract-tracing in male PVCrexAi9 mice revealed a small proportion of PV+ projections from the pIC to the pACC. Electrophysiological analysis confirmed the existence of synaptic inputs into the pACC by pIC GABAergic cells. Optogenetic stimulation of these pathways did not change mechanical nociception, but induced conditioned place preference behavior responses. Our results suggest the presence of inhibitory projections between the pIC and the pACC which are able to selectively modulate affective aspects of neuropathic pain.

【 授权许可】

CC BY   
© Min Zhuo, Bong-Kiun Kaang and BioMed central Ltd. 2023

【 图 表 】

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Fig. 1