Transcriptome and proteome analysis reveals the anti-cancer properties of Hypnea musciformis marine macroalga extract in liver and intestinal cancer cells

Human Genomics

Research

Martina Samiotaki¹  Xiukun Lin²  Rodiola Begolli³  Myrto Chatziangelou³  Dimitrios Stagos³  Antonis Giakountis³  Nikolaos Goutzourelas³  Nikolaos Kollatos³  Sofia Barda³  Andreas Goutas⁴  Varvara Trachana⁵  Paraskevi Malea⁶  Ming Liu⁷  Dimitrios Phaedon Kevrekidis⁸ 

[1] B.S.R.C “Alexander Fleming”, 34 Fleming str, 16672, Vari, Greece;College of Marine Sciences, Beibu Gulf University, 12 Binhai Rd, 535011, Qinzhou, Guangxi, China;Department of Biochemistry and Biotechnology, School of Health Sciences, University of Thessaly, 41500, Biopolis, Larissa, Greece;Department of Biochemistry and Biotechnology, School of Health Sciences, University of Thessaly, 41500, Biopolis, Larissa, Greece;Department of Biology, Faculty of Medicine, University of Thessaly, 41500, Biopolis, Larissa, Greece;Department of Biology, Faculty of Medicine, University of Thessaly, 41500, Biopolis, Larissa, Greece;Department of Botany, School of Biology, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece;Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 266003, Qingdao, China;Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, 266237, Qingdao, China;Laboratory of Forensic Medicine and Toxicology, Department of Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece;

关键词: Hypnea musciformis;    Marine macroalga (seaweed) extracts;    Chemoprevention;    Transcriptomics;    Proteomics;    Liver cancer;    Intestinal cancer;    p53;   

DOI  :  10.1186/s40246-023-00517-0

 received in 2023-06-12, accepted in 2023-07-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMarine seaweeds are considered as a rich source of health-promoting compounds by the food and pharmaceutical industry. Hypnea musciformis is a marine red macroalga (seaweed) that is widely distributed throughout the world, including the Mediterranean Sea. It is known to contain various bioactive compounds, including sulfated polysaccharides, flavonoids, and phlorotannins. Recent studies have investigated the potential anticancer effects of extracts from H. musciformis demonstrating their cytotoxic effects on various cancer cell lines. The anticancer effects of these extracts are thought to be due to the presence of bioactive compounds, particularly sulfated polysaccharides, which have been shown to have anticancer and immunomodulatory effects. However, further studies are needed to fully understand the molecular mechanisms that underlie their anticancer effects and to determine their potential as therapeutic agents for cancer treatment.MethodsH. musciformis was collected from the Aegean Sea (Greece) and used for extract preparation. Transcriptome and proteome analysis was performed in liver and colon cancer human cell lines following treatment with H. musciformis seaweed extracts to characterize its anticancer effect in detail at the molecular level and to link transcriptome and proteome responses to the observed phenotypes in cancer cells.ResultsWe have identified that treatment with the seaweed extract triggers a p53-mediated response at the transcriptional and protein level in liver cancer cells, in contrast to colon cancer cells in which the effects are more associated with metabolic changes. Furthermore, we show that in treated HepG2 liver cancer cells, p53 interacts with the chromatin of several target genes and facilitates their upregulation possibly through the recruitment of the p300 co-activator.ConclusionsOverall, the available evidence suggests that extracts from H. musciformis have the potential to serve as a source of anticancer agents in liver cancer cells mainly through activation of a p53-mediated anti-tumor response that is linked to inhibition of cellular proliferation and induction of cell death.

【 授权许可】

CC BY   
© The Author(s) 2023

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