Effect of proteins from the red seaweed Hypnea musciformis (Wulfen) Lamouroux on the growth of human pathogen yeasts

Brazilian Archives of Biology and Technology

Rossana Aguiar Cordeiro²  Valdirene Moreira Gomes¹  Ana Fontenele Urano Carvalho²  Vânia Maria Maciel Melo² 

[1] ,Universidade Federal do Ceará Departamento de Biologia Fortaleza CE ,Brasil

关键词: Hypnea musciformis;    antifungal activity;    beta-1;    3-glucanase;    chitinase;    lectin;   

DOI  :  10.1590/S1516-89132006000700008

来源: SciELO

【 摘 要 】

A protein fraction, rich in lectin, obtained from the red seaweed Hypnea musciformis by precipitation with ammonium sulfate (F40/70) was screened for chitinase and beta-1,3-glucanase activity and assessed for antifungal potential against the human pathogen yeasts Candida albicans and C. guilliermondii. The F40/70 fraction showed chitinase and beta-1,3-glucanase enzymes, with specific activities of 276.43 and 1880.7 Units.mg -1 protein, respectively. It was capable of inhibiting the growth of C. guilliermondii at the concentrations of 45, 100 and 450 µg protein.ml -1 but it showed only a discrete inhibition against C. albicans irrespective of the tested concentrations. The inhibitory action was shown to be fungistatic and the presence of the glycoprotein fetuin, for which the lectin in the fraction had affinity, abolished the antifungal action. The complete growth recovery following fetuin treatment indicated that chitinase and beta-1,3-glucanase were not involved in the growth inhibition of these yeasts.

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