Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)

Brazilian Archives of Biology and Technology

Felipe Almeida Vieira²  Maura Da Cunha¹  Denise Espellet Klein¹  André De Oliveira Carvalho²  Valdirene Moreira Gomes² 

[1] ,Universidade Estadual Norte Fluminense Darcy Ribeiro Laboratório de fisiologia e Bioquímica de Microrganismos

关键词: beta-1;    3-glucanase;    PR protein;    chitinases;    secretory structure;   

DOI  :  10.1590/S1516-89132006000700004

来源: SciELO

【 摘 要 】

In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2

【 授权许可】

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