Plasma exosomes lncRNA-miRNA-mRNA network construction and its diagnostic efficacy identification in first-episode schizophrenia

BMC Psychiatry

Research

Jinzhi Lv¹  Jianping Feng²  Yong Xu³  Xiaohua Cao⁴  Xiao Wang⁴  Wentao Zhao⁴  Xinrong Li⁴  Yao Gao⁴  Sha Liu⁴  Xinzhe Du⁴  Ruifang Zhang⁵  Zhiyong Ren⁵ 

[1] Department of Physiology, Shanxi Medical University, Taiyuan, China;Department of Psychiatry, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, China;Department of Psychiatry, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, China;Department of Psychiatry, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China;Department of Psychiatry, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, China;Shanxi Key Laboratory of Artificial Intelligence Assisted Diagnosis and Treatment for Mental Disorder, First Hospital of Shanxi Medical University, Taiyuan, China;Female Department of Schizophrenia, Shanxi Province Mental Health Center/Taiyuan Psychiatric Hospital, Taiyuan, China;

关键词: Schizophrenia;    Exosome;    lncRNA;    miRNA;    mRNA;   

DOI  :  10.1186/s12888-023-05052-9

 received in 2022-10-12, accepted in 2023-07-25,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe exosomal lncRNA-miRNA-mRNA networks in first episode schizophrenia (FOS) have not reported yet. This study examined the lncRNA, miRNA and mRNA expression level in exosome derived from first episode schizophrenia (FOS) patients, and explored the the potential of exosomes as biomarkers for schizophrenia.MethodsWe recruited 10 FOS patients and healthy controls (HCs) respectively, examined the lncRNA, miRNA and mRNA expression level of plasma exosome by high throughput sequencing, constructed lncRNA-miRNA-mRNA network, and performed correlation analysis, GO and KEGG pathway analysis, PPI network construction and ROC analysis.ResultsThere were 746 differently expressed lncRNA, 22 differently expressed miRNA, and 2637 differently expressed mRNA in plasma exosome in FOS compared with HCs. Then we constructed ceRNA network consisting of 8 down-regulated lncRNA, 7 up-regulated miRNA and 65 down-regulated mRNA, and 1 up-regulated lncRNA, 1 down-regulated miRNA and 4 up-regulated mRNA. The expression level of 1 lncRNA and 7 mRNA in exosomal network were correlated with PANSS score. GO and KEGG pathway analysis showed that 4 up-regulated mRNAs were enriched in neuropsychiatric system function. Down-regulated mRNA EZH2 and SIRT1 were identified as hub gene. Finally, we detected the ROC curve of ENSG00000251562, miR-26a-5p, EZH2, miR-22-3p, SIRT1, ENSG00000251562—miR-26a-5p—EZH2, ENSG00000251562—miR-22-3p—SIRT1, and found that the AUC of ceRNA network was higher than lncRNA, miRNA and mRNA alone.ConclusionWe constructed the lncRNA-miRNA-mRNA network in exosome derived from FOS plasma, and found that lncRNA-miRNA-mRNA network has potential as biomarkers for FOS.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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