Assessing the susceptibility and efficacy of traditional neurotoxic (pyrethroid) and new-generation insecticides (chlorfenapyr, clothianidin, and pyriproxyfen), on wild pyrethroid-resistant populations of Anopheles gambiae from southern Benin

Malaria Journal

Research

Martin Akogbéto¹  Casimir Kpanou¹  Albert Sourou Salako¹  Aboubakar Sidick¹  Razaki Ossè¹  Saïd Chitou¹  Steve Hougbe¹  Bruno Akinro¹  David Mahouton Zoungbédji²  Germain Gil Padonou²  Alphonse Keller Konkon²  Hermann Sagbohan²  Cyriaque Afoukou³  Rock Aïkpon³  Virgile Gnanguénon⁴  Patrick Condo⁴  Daniel Impoinvil⁵  Ahmed Saadani Hassani⁶ 

[1] Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Benin;Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Benin;Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Godomey, Benin;Programme National de Lutte Contre Le Paludisme, Cotonou, Benin;US President’s Malaria Initiative, US Agency for International Development, Cotonou, Benin;US President’s Malaria Initiative, US Centers for Disease Control and Prevention, Atlanta, USA;US President’s Malaria Initiative, US Centers for Disease Control and Prevention, Cotonou, Benin;

关键词: Chlorfenapyr;    Pyriproxyfen;    Clothianidin;    Anopheles gambiae;    Insecticide resistance;    Long-lasting insecticidal nets;    Bio-efficacy;    Benin;   

DOI  :  10.1186/s12936-023-04664-6

 received in 2023-04-01, accepted in 2023-08-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe objective of this study was to determine the susceptibility of wild Anopheles gambiae sensu lato (s.l.) from southern Benin to the new insecticides (chlorfenapyr (CFP), pyriproxyfen (PPF), and clothianidin (CTD)) and assess the efficacy of insecticide-treated bed nets (ITNs) that contain these new products.MethodsWild An. gambiae from the Benin communes of Allada, Ifangni, Akpro-Missérété, and Porto-Novo were tested for their susceptibility to CFP and PPF using the WHO bottle tests, and pyrethroids (alpha-cypermethrin, deltamethrin, and permethrin) and CTD using WHO tube tests. WHO cone tests were used to evaluate the efficacy of Interceptor® (which contains alpha-cypermethrin (ACM) only), Interceptor® G2, (CFP + ACM), and Royal Guard® nets (PPF + ACM). The ovaries of blood-fed An. gambiae from Ifangni exposed to a new PPF net were dissected, and egg development status was examined using Christopher’s stages to determine the fertility status of the mosquitoes. Using a standardized protocol, the oviposition rate and oviposition inhibition rate were calculated from live blood-fed An. gambiae placed in oviposition chambers after exposure to PPF.ResultsIn all four mosquito populations, pyrethroid mortality ranged from 5 to 80%, while chlorfenapyr and clothianidin mortality ranged from 98 to 100%. At Ifangni, all mosquitoes exposed to Royal Guard® nets were infertile (100%) while the majority (74.9%) of mosquitoes exposed to Interceptor® nets had fully developed their eggs to Christopher’s stage V. The oviposition inhibition rate after exposure of the mosquitoes to the PPF was 99% for the wild population of An. gambiae s.l. and the susceptible laboratory strain, An. gambiae sensu stricto (Kisumu).ConclusionsThe results of this study suggest that pyrethroid-resistant An. gambiae from the selected communes in southern Benin are susceptible to chlorfenapyr, clothianidin, and pyriproxyfen. In addition, based on bioassay results, new and unused Interceptor® G2 and Royal Guard® nets were effective on Ifangni’s mosquito populations. Despite the availability of new effective insecticides, continued vigilance is needed in Benin. Therefore, monitoring of resistance to these insecticides will continue to periodically update the Benin national insecticide resistance database and management plan.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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