Production of a chimeric porcine reproductive and respiratory syndrome virus (PRRSV)-2 vaccine using a lab-scale packed-bed bioreactor CelCradle

BMC Veterinary Research

Research

So-Hyeun Ahn¹  Hwi-Yeon Choi¹  Yeong-Lim Kang¹  Jong-Chul Choi¹  Seung-Yong Park²  Joong-Bok Lee²  Sang-Won Lee²  Chang-Seon Song²  In-Soo Choi² 

[1]Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, 05029, Seoul, Republic of Korea

[2]Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, 05029, Seoul, Republic of Korea

[3]KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, 05029, Seoul, Republic of Korea

关键词: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV);    Vaccine production;    CelCradle;    Packed Bed;    Bioreactor;    Vaccine efficacy;   

DOI  :  10.1186/s12917-023-03659-4

 received in 2021-07-22, accepted in 2023-07-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundWe developed a MARC-145 cell culture and porcine reproductive and respiratory syndrome (PRRS) vaccine production using a novel CelCradle bioreactor. CelCradle is a packed-bed bioreactor capable of both batch and perfusion culture, and the operating parameters are easy to optimize.ResultsIn this study, CelCradle reached a maximum cell density of 8.94 × 105 cells/mL at 5 days post-seeding when seeded at 8.60 × 104 cells/mL (doubling time = 35.52 h). Inoculation of PRRS vaccine candidate, K418DM1.1, was performed at a multiplicity of infection (MOI) of 0.01 at 5 days post-seeding, which resulted in a high viral titer of 2.04 × 108 TCID50/mL and total viral load of 1.02 × 1011 TCID50/500 mL at 2 days post-infection (dpi). The multilayer cultivation system, BioFactory culture, yielded a higher doubling time (37.14 h) and lower viral titer (i.e., 8.15 × 107 TCID50/mL) compared to the CelCradle culture. Thus, the culture medium productivity of the CelCradle culture was 2-fold higher than that of the BioFactory culture. In the animal experiment, the CelCradle-produced vaccine induced high levels of neutralizing antibodies and effectively protected pigs against homologous challenge, as shown by the significantly lower levels of viremia at 1- and 7-days post-challenge (dpc) compared to the non-vaccinated pigs.ConclusionsOverall, this study demonstrates that the CelCradle system is an economical platform for PRRS vaccine production.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023. corrected publication 2023

【 图 表 】

40517_2023_266_Article_IEq66.gif

40517_2023_266_Article_IEq39.gif

40517_2023_266_Article_IEq15.gif

Fig. 2