Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

Journal of Animal Science and Biotechnology

Research

Carlos Alonso-Moreno¹  María Arenas-Moreira¹  Pedro Javier Soria-Meneses²  Alejandro Jurado-Campos²  Ana Josefa Soler²  José Julián Garde²  Virginia Rodríguez-Robledo³  María del Rocío Fernández-Santos³ 

[1] Departamento de Química Inorgánica, Orgánica Y Bioquímica-Centro de Innovación en Química Avanzada (ORFEO-CINQA), Facultad de Farmacia, Universidad de Castilla-La Mancha, 02008, Albacete, Spain;Centro Regional de Investigación Biomédicas, Unidad nanoDrug, Universidad de Castilla-La Mancha, 02008, Albacete, Spain;SaBio IREC (CSIC—UCLM—JCCM), Campus Universitario, S/N, 02071, Albacete, Spain;SaBio IREC (CSIC—UCLM—JCCM), Campus Universitario, S/N, 02071, Albacete, Spain;Facultad de Farmacia, Universidad de Castilla La Mancha, 02071, Albacete, Spain;

关键词: Breeding technology;    Nanoemulsions;    Nanotechnology;    Sperm oxidative stress;    Vitamin E;   

DOI  :  10.1186/s40104-023-00907-3

 received in 2023-02-09, accepted in 2023-06-12,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundArtificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of vitamin E nanoemulsion (NE) for improving sperm quality during transport.ResultsWith the aim of evaluating this proposal, ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60 × 106 spz/mL in Andromed®. Samples containing control and NE (12 mmol/L) with and without exogenous oxidative stress (100 µmol/L Fe2+/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/PI), acrosomal integrity (PNA-FITC/PI), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 h. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples.ConclusionsThe storage of ram spermatozoa in liquid form for 2–5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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