Weissella confusa F213 ameliorated inflammation and maintained intestinal mucosa integrity in chemically induced colitis rats

BMC Research Notes

Research Note

I Gusti Kamasan Nyoman Arijana¹  Ni Nengah Dwi Fatmawati²  Gede Ngurah Rsi Suwardana²  Ida Ayu Gde Wahyudevi Dharmika²  I Ketut Mariadi³  Ida Bagus Oka Winaya⁴  Yan Ramona⁵  Komang Ayu Nocianitri⁶  I Nengah Sujaya⁷ 

[1] Department of Histology, Faculty of Medicine, Udayana University, Bali, Indonesia;Department of Microbiology, Faculty of Medicine, Udayana University, Bali, Indonesia;Division Gastroenterohepatology, Department of Internal Medicine, Udayana University/Professor Dr. I.G.N.G. Ngoerah Hospital, Denpasar, Bali, Indonesia;Pathology Anatomy Laboratory, Faculty of Veterinary, Udayana University, Bali, Indonesia;School of Biology, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia;School of Food Technology, Faculty of Agricultural Technology, Udayana University, Bali, Indonesia;School of Public Health, Faculty of Medicine, Udayana University, Bali, Indonesia;

关键词: Probiotics;    Weissella confusa;    Colitis;    Inflammation;    Mucosa integrity;   

DOI  :  10.1186/s13104-023-06456-2

 received in 2023-05-13, accepted in 2023-08-10,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectiveThis study was performed to investigate the potential effects of Weissella confusa F213 (WCF213) on chemically-induced colitis rats. Twelve male Wistar rats were divided into three groups: T1 (saline sterile), T2 (2.5% dextran sulfate sodium (DSS)- for 7 days), and T3 (WCF213 for 14 days, continued with 2.5% DSS for 7 days). The disease activity index (DAI) was monitored. After sacrificing the rats, the colon was collected for length measurement, local TNF-α level, HE staining for histology, and ZO-1 expression by using immunohistochemistry.ResultsWCF213 administration prevented weight loss and haematochezia, maintained average colon length and alleviated the clinical symptom of colitis, such as diarrhoea, albeit statistically non-significant (p < 0.05) compared with the T2 group. The histopathology of WCF213-treated colitis rats showed better architecture and less inflammatory cell infiltration into colon tissue. WCF213 significantly maintained the expression of ZO-1 in the mucosa (p < 0.001) and markedly reduced mucosal TNF-α concentration (p < 0.001) compared with the DSS group. Hence, these findings suggested that WCF213 attenuated clinical symptoms and inflammation and maintained mucosal integrity in DSS-induced colitis in vivo.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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