Identification of QTL for kernel weight and size and analysis of the pentatricopeptide repeat (PPR) gene family in cultivated peanut (Arachis hypogaea L.)

BMC Genomics

Research

Xinyou Zhang¹  Yuanjin Fang¹  Feiyan Qi²  Hua Liu²  Li Qin²  Ziqi Sun²  Wenzhao Dong²  Bingyan Huang²  Jihua Wu³ 

[1] College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, China;Henan Academy of Agricultural Sciences/Henan Institute of Crop Molecular Breeding/Shennong Laboratory/Key Laboratory of Oil Crops in Huang-Huai-Hai Planis, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement, 450002, Zhengzhou, China;Henan Academy of Agricultural Sciences/Henan Institute of Crop Molecular Breeding/Shennong Laboratory/Key Laboratory of Oil Crops in Huang-Huai-Hai Planis, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement, 450002, Zhengzhou, China;Shangqiu Academy of Agriculture and Forestry, 476002, Shangqiu, China;

关键词: Arachis hypogaea;    Kernel weight;    Kernel size;    QTLs (quantitative trait loci);    Pentatricopeptide repeat (PPR);   

DOI  :  10.1186/s12864-023-09568-y

 received in 2023-06-08, accepted in 2023-08-09,  发布年份 2023

来源: Springer

【 摘 要 】

Peanut (Arachis hypogaea L.) is an important oilseed crop worldwide. Improving its yield is crucial for sustainable peanut production to meet increasing food and industrial requirements. Deciphering the genetic control underlying peanut kernel weight and size, which are essential components of peanut yield, would facilitate high-yield breeding. A high-density single nucleotide polymorphism (SNP)-based linkage map was constructed using a recombinant inbred lines (RIL) population derived from a cross between the variety Yuanza9102 and a germplasm accession wt09-0023. Kernel weight and size quantitative trait loci (QTLs) were co-localized to a 0.16 Mb interval on Arahy07 using inclusive composite interval mapping (ICIM). Analysis of SNP, and Insertion or Deletion (INDEL) markers in the QTL interval revealed a gene encoding a pentatricopeptide repeat (PPR) superfamily protein as a candidate closely linked with kernel weight and size in cultivated peanut. Examination of the PPR gene family indicated a high degree of collinearity of PPR genes between A. hypogaea and its diploid progenitors, Arachis duranensis and Arachis ipaensis. The candidate PPR gene, Arahy.JX1V6X, displayed a constitutive expression pattern in developing seeds. These findings lay a foundation for further fine mapping of QTLs related to kernel weight and size, as well as validation of candidate genes in cultivated peanut.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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