Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B

Proteome Science

Research

Yu Xie¹  Meiyan Luo¹  Lili Wu²  Gang Qin²  Peng Zhu²  Wen Chen³  Gang Wu³  Bo Wang³  Yunjian Sheng³  Qian Zhang³  Cunliang Deng³ 

[1] College of Graduate, Southwest Medical University, 646000, Luzhou, Sichuan, China;Department of Gastroenterology, Suining First Pepole’s Hospital, 629000, Suining, Sichuan, China;Department of Infectious Diseases, The Affiliated Hospital of Southwest Medical University, 646000, Luzhou, Sichuan, China;

关键词: Chronic hepatitis B;    Hepatitis B virus;    CHB;    DIA-MS;   

DOI  :  10.1186/s12953-023-00209-6

 received in 2023-02-25, accepted in 2023-05-29,  发布年份 2023

来源: Springer

【 摘 要 】

Chronic hepatitis B is a significant public health problem and complex pathologic process, and unraveling the underlying mechanisms and pathophysiology is of great significance. Data independent acquisition mass spectrometry (DIA-MS) is a label-free quantitative proteomics method that has been successfully applied to the study of a wide range of diseases. The aim of this study was to apply DIA-MS for proteomic analysis of patients with chronic hepatitis B. We performed comprehensive proteomics analysis of protein expression in serum samples from HBV patients and healthy controls by using DIA-MS. Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein network analysis were performed on differentially expressed proteins and were further combined with literature analysis. We successfully identified a total of 3786 serum proteins with a high quantitative performance from serum samples in this study. We identified 310 differentially expressed proteins (DEPs) (fold change > 1.5 and P value < 0.05 as the criteria for a significant difference) between HBV and healthy samples. A total of 242 upregulated proteins and 68 downregulated proteins were among the DEPs. Some protein expression levels were significantly elevated or decreased in patients with chronic hepatitis B, indicating a relation to chronic liver disease, which should be further investigated.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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