The microbiome of Riccia liverworts is an important reservoir for microbial diversity in temporary agricultural crusts

Environmental Microbiome

Research

Christian Berg¹  Martina Pöltl¹  Bettina Semler²  Wisnu Adi Wicaksono²  Tomislav Cernava³  Gabriele Berg⁴ 

[1] Institute of Biology, University of Graz, 8010, Graz, Austria;Institute of Environmental Biotechnology, Graz University of Technology, 8010, Graz, Austria;Institute of Environmental Biotechnology, Graz University of Technology, 8010, Graz, Austria;Graz University of Technology, Graz, Austria;Institute of Environmental Biotechnology, Graz University of Technology, 8010, Graz, Austria;Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Potsdam, Germany;Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany;

关键词: Bacteria;    Fungi;    Methylobacteria;    Liverworts;    Microbiome;    Crop history;   

DOI  :  10.1186/s40793-023-00501-0

 received in 2023-01-06, accepted in 2023-05-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe microbiota of liverworts provides an interesting model for plant symbioses; however, their microbiome assembly is not yet understood. Here, we assessed specific factors that shape microbial communities associated with Riccia temporary agricultural crusts in harvested fields by investigating bacterial, fungal and archaeal communities in thalli and adhering soil from different field sites in Styria and Burgenland, Austria combining qPCR analyses, amplicon sequencing and advanced microscopy.ResultsRiccia spec. div. was colonized by a very high abundance of bacteria (1010 16S rRNA gene copies per g of thallus) as well as archaea and fungi (108 ITS copies per g of thallus). Each Riccia thallus contain approx. 1000 prokaryotic and fungal ASVs. The field type was the main driver for the enrichment of fungal taxa, likely due to an imprint on soil microbiomes by the cultivated crop plants. This was shown by a higher fungal richness and different fungal community compositions comparing liverwort samples collected from pumpkin fields, with those from corn fields. In contrast, bacterial communities linked to liverworts are highly specialized and the soil attached to them is not a significant source of these bacteria. Specifically, enriched Cyanobacteria, Bacteroidetes and Methylobacteria suggest a symbiotic interaction. Intriguingly, compared to the surrounding soil, the thallus samples were shown to enrich several well-known bacterial and fungal phytopathogens indicating an undescribed role of liverworts as potential reservoirs of crop pathogens.ConclusionsOur results provide evidence that a stable bacterial community but varying fungal communities are colonizing liverwort thalli. Post-harvest, temporary agricultural biocrusts are important reservoirs for microbial biodiversity but they have to be considered as potential reservoirs for pathogens as well.

【 授权许可】

CC BY   
© The Author(s) 2023

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