Mechanochemical-assisted extraction and enzymatic hydrolysis of calcium phytate from defatted rice bran

Chemical and Biological Technologies in Agriculture

Research

XingYi Zhu¹  Weike Su¹  Lei Zheng²  Jialu Wang³ 

[1] Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China;School of Laboratory Medicine and Bioengineering, Hangzhou Medical College, 310053, Hangzhou, People’s Republic of China;Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China;School of Nursing, Zhejiang Chinese Medical University, 310053, Hangzhou, People’s Republic of China;Department of Orthopaedics, Sir Run Run Shaw Hospital, 310016, Hangzhou, People’s Republic of China;

关键词: Mechanochemistry;    Extraction;    Enzymatic hydrolysis;    Calcium phytate;    Rice bran;   

DOI  :  10.1186/s40538-023-00424-5

 received in 2023-04-07, accepted in 2023-06-01,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMechanochemical-assisted extraction (MCAE) and mechanochemical-assisted enzyme catalysis (MCAEC) methods were developed for extraction and enzymatic hydrolysis of calcium phytate from defatted rice bran, respectively.ResultsThe MCAE conditions were optimized by response surface methodology: 10% sodium carbonate as the solid reagent, 33 min grinding time, 18:1 ball–material ratio, 330 rpm ball milling speed, and pH 5.5 extraction. Under these conditions, the yield of calcium phytate was 97.0 mg/g. The MCAEC conditions were optimized as follows: 2% enzyme addition, MM400 milling time 36 min, PM400 milling speed 262 rpm, MM400 milling frequency 27 Hz, and the addition of pH 5.5 sodium acetate buffer 3 mL. Under these conditions, the hydrolysis rate of calcium phytate was 39.7%.ConclusionCompared with traditional extraction and enzymatic hydrolysis methods, MCAE and MCAEC could obtain higher yields and hydrolysis rates, respectively.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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