Proteomic and functional analysis of HDL subclasses in humans and rats: a proof-of-concept study

Lipids in Health and Disease

Research

Hong Wang¹  Jinlan Bao²  Canxia Huang³  Qiaofei Chen⁴  Yuling Zhang⁴  Jingjing Huang⁴  Jie Zhang⁴  Runlu Sun⁴  Kexin Wen⁴  Xiaoying Wu⁴  Hongwei Li⁴  Zhijian He⁴  Xinyi Huang⁴  Yue Wang⁴  Ayiguli Abudukeremu⁴ 

[1]Centers for Metabolic & Cardiovascular Research, Department of Pharmacology, Temple University School of Medicine, 19140, Philadelphia, PA, USA

[2]Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 510120, Guangzhou, China

[3]Comprehensive Department, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 510120, Guangzhou, China

[4]Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 510120, Guangzhou, China

[5]Critical Care Medicine Department, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 510120, Guangzhou, China

[6]Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 510120, Guangzhou, China

[7]Department of Cardiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 510120, Guangzhou, China

关键词: High density lipoprotein;    HDL subclass;    Proteomics;    Antioxidation;    Cholesterol efflux;    Fast protein liquid chromatography;   

DOI  :  10.1186/s12944-023-01829-9

 received in 2022-12-07, accepted in 2023-05-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe previous study investigated whether the functions of small, medium, and large high density lipoprotein (S/M/L-HDL) are correlated with protein changes in mice. Herein, the proteomic and functional analyses of high density lipoprotein (HDL) subclasses were performed in humans and rats.MethodsAfter purifying S/M/L-HDL subclasses from healthy humans (n = 6) and rats (n = 3) using fast protein liquid chromatography (FPLC) with calcium silica hydrate (CSH) resin, the proteomic analysis by mass spectrometry was conducted, as well as the capacities of cholesterol efflux and antioxidation was measured.ResultsOf the 120 and 106 HDL proteins identified, 85 and 68 proteins were significantly changed in concentration among the S/M/L-HDL subclasses in humans and rats, respectively. Interestingly, it was found that the relatively abundant proteins in the small HDL (S-HDL) and large HDL (L-HDL) subclasses did not overlap, both in humans and in rats. Next, by searching for the biological functions of the relatively abundant proteins in the HDL subclasses via Gene Ontology, it was displayed that the relatively abundant proteins involved in lipid metabolism and antioxidation were enriched more in the medium HDL (M-HDL) subclass than in the S/L-HDL subclasses in humans, whereas in rats, the relatively abundant proteins associated with lipid metabolism and anti-oxidation were enriched in M/L-HDL and S/M-HDL, respectively. Finally, it was confirmed that M-HDL and L-HDL had the highest cholesterol efflux capacity among the three HDL subclasses in humans and rats, respectively; moreover, M-HDL exhibited higher antioxidative capacity than S-HDL in both humans and rats.ConclusionsThe S-HDL and L-HDL subclasses are likely to have different proteomic components during HDL maturation, and results from the proteomics-based comparison of the HDL subclasses may explain the associated differences in function.

【 授权许可】

CC BY   
© The Author(s) 2023

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