Completing the view – histologic insights from circular AAA specimen including 3D imaging

Diagnostic Pathology

Research

Johannes R. Müller¹  Anna-Leonie Menges²  Alexander Zimmermann²  Jaroslav Pelisek²  Gabor Biro³  Marie-Luise Engl³  Renate Hegenloh³  Albert Busch⁴  Hans-Henning Eckstein⁵  Nadja Sachs⁵  Maja Nackenhorst⁶  Christian Reeps⁷  Anja Hofmann⁷  Derek Magee⁸  Ellen Geibelt⁹  Martin Falk^1,10 

[1] DFG Cluster of Excellence “Physics of Life”, TU Dresden, Dresden, Germany;Department for Vascular Surgery, University Hospital Zurich, Zurich, Switzerland;Department for Vascular and Endovascular Surgery, Klinikum Rechts Der Isar, Technical University Dresden, Dresden, Germany;Department for Vascular and Endovascular Surgery, Klinikum Rechts Der Isar, Technical University Dresden, Dresden, Germany;Division of Vascular and Endovascular Surgery, Department for Visceral-, Thoracic and Vascular Surgery, Medical Faculty Carl Gustav Carus and University Hospital, Technical University Munich, Fetscherstrasse 74, 01307, Munich, Germany;Department for Vascular and Endovascular Surgery, Klinikum Rechts Der Isar, Technical University Dresden, Dresden, Germany;German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Berlin, Germany;Department of Pathology, Medical University of Vienna, Vienna, Austria;Division of Vascular and Endovascular Surgery, Department for Visceral-, Thoracic and Vascular Surgery, Medical Faculty Carl Gustav Carus and University Hospital, Technical University Munich, Fetscherstrasse 74, 01307, Munich, Germany;HeteroGenius Limited, Leeds, UK;School of Computing, University of Leeds, Leeds, UK;Light Microscopy Facility, Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden, Dresden, Germany;Scientific Visualization Group, Department of Science and Technology (ITN), Linköping University, Linköping, Sweden;

关键词: Abdominal Aortic Aneurysm;    Aneurysm sac;    3D histology;    Inflammation;   

DOI  :  10.1186/s13000-023-01359-z

 received in 2023-02-12, accepted in 2023-05-23,  发布年份 2023

来源: Springer

【 摘 要 】

Abdominal aortic aneurysm (AAA) is a pathologic enlargement of the infrarenal aorta with an associated risk of rupture. However, the responsible mechanisms are only partially understood. Based on murine and human samples, a heterogeneous distribution of characteristic pathologic features across the aneurysm circumference is expected. Yet, complete histologic workup of the aneurysm sac is scarcely reported. Here, samples from five AAAs covering the complete circumference partially as aortic rings are investigated by histologic means (HE, EvG, immunohistochemistry) and a new method embedding the complete ring. Additionally, two different methods of serial histologic section alignment are applied to create a 3D view. The typical histopathologic features of AAA, elastic fiber degradation, matrix remodeling with collagen deposition, calcification, inflammatory cell infiltration and thrombus coverage were distributed without recognizable pattern across the aneurysm sac in all five patients. Analysis of digitally scanned entire aortic rings facilitates the visualization of these observations. Immunohistochemistry is feasible in such specimen, however, tricky due to tissue disintegration. 3D image stacks were created using open-source and non-generic software correcting for non-rigid warping between consecutive sections. Secondly, 3D image viewers allowed visualization of in-depth changes of the investigated pathologic hallmarks. In conclusion, this exploratory descriptive study demonstrates a heterogeneous histomorphology around the AAA circumference. Warranting an increased sample size, these results might need to be considered in future mechanistic research, especially in reference to intraluminal thrombus coverage. 3D histology of such circular specimen could be a valuable visualization tool for further analysis.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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