Glycyrrhinic acid and probiotics alleviate deoxynivalenol-induced cytotoxicity in intestinal epithelial cells

AMB Express

Original Article

Chaoqi Liu¹  Qingqiang Yin¹  Juan Chang¹  Ping Wang¹  Ting Zhou²  Guorong Yan³  Xiaoxiang Xu⁴ 

[1] College of Animal Science and Technology, Henan Agricultural University, 450046, Zhengzhou, China;Guelph Research and Development Centre, Agriculture and Agri-Food Canada, N1G 5C9, Guelph, ON, Canada;Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 200443, Shanghai, China;Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 200443, Shanghai, China;College of Animal Science and Technology, Henan Agricultural University, 450046, Zhengzhou, China;

关键词: Deoxynivalenol;    Glycyrrhinic acid;    Compound probiotics;    Inflammation;    IPEC-J2 cells;   

DOI  :  10.1186/s13568-023-01564-5

 received in 2022-09-27, accepted in 2023-05-18,  发布年份 2023

来源: Springer

【 摘 要 】

Deoxynivalenol (DON) is one of the most prevalent mycotoxin contaminants, which posing a serious health threat to animals and humans. Previous studies have found that individually supplemented probiotic or glycyrrhinic acid (GA) could degrade DON and alleviate DON-induced cytotoxicity. The present study investigated the effect of combining GA with Saccharomyces cerevisiae (S. cerevisiae) and Enterococcus faecalis (E. faecalis) using orthogonal design on alleviating IPEC-J2 cell damage induced by DON. The results showed that the optimal counts of S. cerevisiae and E. faecalis significantly promoted cell viability. The optimal combination for increasing cell viability was 400 µg/mL GA, 1 × 106 CFU/mL S. cerevisiae and 1 × 106 CFU/mL E. faecalis to make GAP, which not only significantly alleviated the DON toxicity but also achieved the highest degradation rate of DON (34.7%). Moreover, DON exposure significantly increased IL-8, Caspase3 and NF-κB contents, and upregulated the mRNA expressions of Bax, Caspase 3, NF-κB and the protein expressions of Bax, TNF-α and COX-2. However, GAP addition significantly reduced aforementioned genes and proteins. Furthermore, GAP addition significantly increased the mRNA expressions of Claudin-1, Occludin, GLUT2 and ASCT2, and the protein expressions of ZO-1, Claudin-1 and PePT1. It was inferred that the combination of GA, S. cerevisiae, and E. faecalis had the synergistic effect on enhancing cell viability and DON degradation, which could protect cells from DON-induced damage by reducing DON cytotoxicity, alleviating cell apoptosis and inflammation via inhibiting NF-κB signaling pathway, improving intestinal barrier function, and regulating nutrient absorption and transport. These findings suggest that GAP may have potential as a dietary supplement for livestock or humans exposed to DON-contaminated food or feed.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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Fig. 4

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