A novel colloidal gold labeled antigen for the detection of Deoxynivalenol using an immunochromatographic assay method

2017 3rd International Conference on Energy, Environment and Materials Science

能源学;生态环境科学;材料科学

Jin, Yu^1 ; Liu, Renrong^1 ; Zhu, Lixin^1 ; Chen, Zhenzhen^1

School of Life Science, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi

330013, China^1

关键词: Colloidal gold;    Control lines;    Deoxynivalenol;    Immunochromatographic;    Immunochromatographic assays;    Matrix interference;    Rapid detection;    Test-lines;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/94/1/012010/pdf
DOI  :  10.1088/1755-1315/94/1/012010

来源: IOP

【 摘 要 】

In this paper, an immunochromatographic assay card was developed for the detection of DON in feed and cereals using a novel colloidal gold labeling method. For the colloidal gold immunochromatographic rapid detection (GICD) card, a monoclonal antibody DON-mAb and a goat anti-chicken IgY were drawn on NC membrane as the test line (T line) and the control line (C line) respectively. A gold labeled DON-CBSA conjugate and a gold labeled chicken IgY were sprayed onto the conjugate pad. The GICD card has cut-off levels of 50ng/mL for DON, which is invulnerable to matrix interference, and applicable to a wide range of samples. The GICD detecting results of feed and grain samples were compared with the results of ELISA testing, which showed good consistency.