Colloidal gold labelling of l,4‐β‐D‐glucan cellobiohydrolase adsorbed on cellulose substrates

FEBS Letters

Henrissat, B.¹  Vuong, R.¹  Chanzy, H.¹ 

[1] Centre de Recherches sur les Macromolécules Végétales (CNRS), BP 68, 38402 Saint-Martin-d'Heres, France

关键词: Colloidal gold;    Enzyme labelling;    1;    4-β-D-Glucan cellobiohydrolase;    (Trichoderma reesei);    Adsorption;    Cellulose;    Electron microscopy;   

DOI  :  10.1016/0014-5793(84)81124-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 1,4-β-D-glucan cellobiohydrolase I (CBHI) from Trichoderma reesei was labelled with 4–6 nm gold particles. This CBHI-gold complex still retains 60% of the original CBHI activity and allows good visualization of the enzyme adsorbed at the surface of cellulose mirofibrils or microcrystals. The main features of the adsorption are: rapid and irreversible binding; preference of the crystal edges instead of the crystal surface for the binding; the absence of specificity for the crystal tips where the cellulose chain ends are supposedly located.

【 授权许可】

Unknown