Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy

Microbiome

Research

Jie Gu¹  Xun Qian¹  Yang Liu²  Shuo Jiao²  Gehong Wei²  Hao Wang²  Xihui Shen²  Weimin Chen²  Shiheng Tao² 

[1] Interdisciplinary Research Center for Soil Microbial Ecology and Land Sustainable Productivity in Dry Areas, Northwest A&F University, 712100, Yangling, Shaanxi, People’s Republic of China;College of Natural Resources and Environment, Northwest A&F University, 712100, Yangling, Shaanxi, People’s Republic of China;State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F University, 3 Taicheng Road, 712100, Yangling, Shaanxi, People’s Republic of China;

关键词: Autotoxicity;    Allelochemical;    Rhizosphere;    Continuous cropping obstacle;    Glycyrrhiza uralensis;   

DOI  :  10.1186/s40168-023-01511-3

 received in 2022-04-25, accepted in 2023-03-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAllelopathy is closely associated with rhizosphere biological processes, and rhizosphere microbial communities are essential for plant development. However, our understanding of rhizobacterial communities under influence of allelochemicals in licorice remains limited. In the present study, the responses and effects of rhizobacterial communities on licorice allelopathy were investigated using a combination of multi-omics sequencing and pot experiments, under allelochemical addition and rhizobacterial inoculation treatments.ResultsHere, we demonstrated that exogenous glycyrrhizin inhibits licorice development, and reshapes and enriches specific rhizobacteria and corresponding functions related to glycyrrhizin degradation. Moreover, the Novosphingobium genus accounted for a relatively high proportion of the enriched taxa and appeared in metagenomic assembly genomes. We further characterized the different capacities of single and synthetic inoculants to degrade glycyrrhizin and elucidated their distinct potency for alleviating licorice allelopathy. Notably, the single replenished N (Novosphingobium resinovorum) inoculant had the greatest allelopathy alleviation effects in licorice seedlings.ConclusionsAltogether, the findings highlight that exogenous glycyrrhizin simulates the allelopathic autotoxicity effects of licorice, and indigenous single rhizobacteria had greater effects than synthetic inoculants in protecting licorice growth from allelopathy. The results of the present study enhance our understanding of rhizobacterial community dynamics during licorice allelopathy, with potential implications for resolving continuous cropping obstacle in medicinal plant agriculture using rhizobacterial biofertilizers.9UbUWYdP6gv58ro7UNXMiqVideo Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 4

40517_2023_258_Article_IEq129.gif

Fig. 6

41116_2023_36_Article_IEq816.gif

Fig. 2