Metagenomic analysis reveals specific BTEX degrading microorganisms of a bacterial consortium

AMB Express

Original Article

Xian-yuan Du¹  Jia-yu Song¹  Hui-jun Wu¹  Jin Zheng¹  Jia-cai Xie¹  Wen-jing Wu² 

[1] State Key Laboratory of Petroleum Pollution Control, National Petroleum Corporation Research Institute of Safety and Environmental Technology, 102206, Beijing, China;State Key Laboratory of Petroleum Pollution Control, National Petroleum Corporation Research Institute of Safety and Environmental Technology, 102206, Beijing, China;College of Life Science, Northwest University, 710000, Xian, China;

关键词: Petroleum hydrocarbon;    Microbial consortium;    Cyclohexane biodegradation;    BTEX biodegradation;    Metagenomic analysis;   

DOI  :  10.1186/s13568-023-01541-y

 received in 2022-11-17, accepted in 2023-03-23,  发布年份 2023

来源: Springer

【 摘 要 】

Petroleum hydrocarbon contamination is of environmental and public health concerns due to its toxic components. Bioremediation utilizes microbial organisms to metabolism and remove these contaminants. The aim of this study was to enrich a microbial community and examine its potential to degrade petroleum hydrocarbon. Through successive enrichment, we obtained a bacterial consortium using crude oil as sole carbon source. The 16 S rRNA gene analysis illustrated the structural characteristics of this community. Metagenomic analysis revealed the specific microbial organisms involved in the degradation of cyclohexane and all the six BTEX components, with a demonstration of the versatile metabolic pathways involved in these reactions. Results showed that our consortium contained the full range of CDSs that could potentially degrade cyclohexane, benzene, toluene, and (o-, m-, p-) xylene completely. Interestingly, a single taxon that possessed all the genes involved in either the activation or the central intermediates degrading pathway was not detected, except for the Novosphingobium which contained all the genes involved in the upper degradation pathway of benzene, indicating the synergistic interactions between different bacterial genera during the hydrocarbon degradation.

【 授权许可】

CC BY   
© The Author(s) 2023

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