Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture

Biological research: BR

Suyue Li¹  Hongyu Li²  Lei Yan³  Peng Chen⁴  Ningbo Wang⁵  Ning Liang⁶  Yuxia Wang⁶  Xiaojuan Yan⁶  Yiqing Wang⁶ 

[1] College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, People’Gansu Institute of Business and Technology, Lanzhou, People’Key Laboratory of Fermentation Resources and Application of Institutes of Higher Learning in Sichuan, School of Life Science and Food Engineering, Institute for Bioengeering, Yibin University, Yibin, People’School of Pharmacy, Lanzhou University, Lanzhou, People’The Reproductive Medicine Research Center of the First Hospital of Lanzhou University, Lanzhou, People’s Republic of China

关键词: Renewable Energy;    Biohydrogen;    Microbial consortium;    Hydrogen;   

DOI  :  10.1186/s40659-015-0015-x

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

The development of clean or novel alternative energy has become a global trend that will shape the future of energy. In the present study, 3 microbial strains with different oxygen requirements, including Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. The effects of metal ions, organic acids and carbohydrate substrates on this system were analyzed and compared using electrochemical and kinetic assays. It was then tested using small-scale experiments to evaluate its ability to convert starch in 5 L of organic wastewater into hydrogen. For the one-step biohydrogen production experiment, H1 medium (nutrient broth and potato dextrose broth) was mixed directly with GAM broth to generate H2 medium (H1 medium and GAM broth). Finally, Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D of three species microbial co-culture to produce hydrogen under anaerobic conditions. For the two-step biohydrogen production experiment, the H1 medium, after cultured the microbial strains Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, was centrifuged to remove the microbial cells and then mixed with GAM broth (H2 medium). Afterward, the bacterial strain Clostridium acetobutylicum ATCC 824 was inoculated into the H2 medium to produce hydrogen by anaerobic fermentation. The experimental results demonstrated that the optimum conditions for the small-scale fermentative hydrogen production system were at pH 7.0, 35°C, a mixed medium, including H1 medium and H2 medium with 0.50 mol/L ferrous chloride, 0.50 mol/L magnesium sulfate, 0.50 mol/L potassium chloride, 1% w/v citric acid, 5% w/v fructose and 5% w/v glucose. The overall hydrogen production efficiency in the shake flask fermentation group was 33.7 mL/h-1.L-1, and those the two-step and the one-step processes of the small-scale fermentative hydrogen production system were 41.2 mL/h-1.L-1 and 35.1 mL/h-1.L-1, respectively. Therefore, the results indicate that the hydrogen production efficiency of the two-step process is higher than that of the one-step process.

【 授权许可】

CC BY