Electromagnetic fields ameliorate hepatic lipid accumulation and oxidative stress: potential role of CaMKKβ/AMPK/SREBP-1c and Nrf2 pathways

BioMedical Engineering OnLine

Research

Juan Liu¹  Yuanzhe Li¹  Kangning Xie¹  Chenxu Zhang¹  Jinxiu Cui¹  Erping Luo¹  Chi Tang²  Mingming Zhai² 

[1] Department of Biomedical Engineering, Fourth Military Medical University, No. 169 Changle West Road, 710032, Xi’an, China;Department of Biomedical Engineering, Fourth Military Medical University, No. 169 Changle West Road, 710032, Xi’an, China;Shaanxi Provincial Key Laboratory of Bioelectromagnetic Detection and Intelligent Perception, Xi’an, China;

关键词: NAFLD;    Electromagnetic fields;    Lipid metabolism;    AMPK;    Oxidative stress;    Nrf2;   

DOI  :  10.1186/s12938-023-01114-x

 received in 2023-01-11, accepted in 2023-05-13,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundNonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, and is related to disturbed lipid metabolism and redox homeostasis. However, a definitive drug treatment has not been approved for this disease. Studies have found that electromagnetic fields (EMF) can ameliorate hepatic steatosis and oxidative stress. Nevertheless, the mechanism remains unclear.MethodsNAFLD models were established by feeding mice a high-fat diet. Simultaneously, EMF exposure is performed. The effects of the EMF on hepatic lipid deposition and oxidative stress were investigated. Additionally, the AMPK and Nrf2 pathways were analysed to confirm whether they were activated by the EMF.ResultsExposure to EMF decreased the body weight, liver weight and serum triglyceride (TG) levels and restrained the excessive hepatic lipid accumulation caused by feeding the HFD. The EMF boosted CaMKKβ protein expression, activated AMPK phosphorylation and suppressed mature SREBP-1c protein expression. Meanwhile, the activity of GSH-Px was enhanced following an increase in nuclear Nrf2 protein expression by PEMF. However, no change was observed in the activities of SOD and CAT. Consequently, EMF reduced hepatic reactive oxygen species (ROS) and MDA levels, which means that EMF relieved liver damage caused by oxidative stress in HFD-fed mice.ConclusionsEMF may activate the CaMKKβ/AMPK/SREBP-1c and Nrf2 pathways to control hepatic lipid deposition and oxidative stress. This investigation indicates that EMF may be a novel therapeutic method for NAFLD.

【 授权许可】

CC BY   
© The Author(s) 2023

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