Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast

BMC Microbiology

Research

Camelia Diguță¹  Florentina Matei¹  Wahauwouélé Hermann Coulibaly²  Fatoumata Camara³  N’goran Richard Kouadio³ 

[1] Applied Microbiology Laboratory, Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine Bucharest, 59 Mărăsti Blvd, 011464, Bucharest, Romania;Biotechnology and Food Microbiology Laboratory, Food Science and Technology, Formation and Research Unit, University Nangui Abrogoua, 02 BP 801 Abidjan 02, Abidjan, Côte d’Ivoire;Applied Microbiology Laboratory, Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine Bucharest, 59 Mărăsti Blvd, 011464, Bucharest, Romania;Nutrition and Food Safety Laboratory, Food Science and Technology, Formation and Research Unit, University Nangui Abrogoua, 02 BP 801 Abidjan 02, Abidjan, Côte d’Ivoire;

关键词: Tilapia fish;    Pediococcus acidilactici;    Pediococcus pentosaceus;    Lactobacillus plantarum;    Probiotics;    Aquaculture;   

DOI  :  10.1186/s12866-023-02899-6

 received in 2022-04-06, accepted in 2023-05-17,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundProbiotics have recently been applied in aquaculture as eco-friendly alternatives to antibiotics to improve fish health, simultaneously with the increase of production parameters. The present study aimed to investigate the functional potential of lactic acid bacteria (LAB) isolated from the gut of Tilapia (Oreochromis niloticus) originating from the aquaculture farm of Oceanologic Research Center in Ivory Coast.ResultsTwelve LAB strains were identified by 16 S rDNA gene sequence homology analysis belonging to two genera Pediococcus (P. acidilactici and P. pentosaceus) and Lactobacillus (L. plantarum) with a predominance of P. acidilactici. Several aspects including functional, storage, and safety characteristics were taken into consideration in the selection process of the native LAB isolates as potential probiotics. All LAB isolates showed high antagonistic activity against bacterial pathogens like Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus. In addition, the LAB isolates exhibited different degrees of cell surface hydrophobicity in the presence of hexane, xylene, and chloroform as solvents and a good ability to form biofilm. The strong antioxidant activity expressed through the DPPH scavenging capacity of LAB intact cells and their cell-free supernatants was detected. LAB strains survived between 34.18% and 49.9% when exposed to low pH (1.5) and pepsin for 3 h. In presence of 0.3% bile salts, the growth rate ranged from 0.92 to 21.46%. Antibiotic susceptibility pattern of LAB isolates showed sensitivity or intermediate resistance to amoxicillin, cephalothin, chloramphenicol, imipenem, kanamycin, penicillin, rifampicin, streptomycin, tetracycline and resistance to oxacillin, gentamicin, and ciprofloxacin. No significant difference in antibiotic susceptibility pattern was observed between P. acidilactici and P. pentosaceus strains. The non-hemolytic activity was detected. Following the analysis of the enzyme profile, the ability of LAB isolates to produce either lipase or β-galactosidase or both enzymes was highlighted. Furthermore, the efficacy of cryoprotective agents was proved to be isolate-dependent, with LAB isolates having a high affinity for D-sorbitol and sucrose.ConclusionThe explored LAB strains inhibited the growth of pathogens and survived after exposure to simulated gastrointestinal tract conditions. The safety and preservative properties are desirable attributes of these new probiotic strains hence recommended for future food and feed applications.

【 授权许可】

CC BY   
© The Author(s) 2023

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