Annals of Clinical Microbiology and Antimicrobials | |
A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections | |
Research | |
Yueling Xiong1  Xiaolei Tang1  Feng Hu2  Ying Hua3  Fan Su4  Yufeng Wen4  Jian Hu4  Xia Ren4  | |
[1] Centre of Translational Medicine and Vascular Disease Research Center, The Second Affiliated Hospital of Wannan Medical College, Kangfu Road 10#, Jinghu District, 241000, Wuhu, Anhui, China;Department of Blood Transfusion, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), 241000, Wuhu, Anhui, China;School of Nursing, Wannan Medical College, 241000, Wuhu, Anhui, China;School of Public Health, Wannan Medical College, No.22, Wenchang Xi Road, 241002, Wuhu, Anhui, China; | |
关键词: Invasive fungal infections (IFI); Aptamers; Systematic Evolution of Ligands by EXponential enrichment; Self-assembling; Visual diagnosis; G-quadruplex; | |
DOI : 10.1186/s12941-023-00570-6 | |
received in 2022-10-25, accepted in 2023-02-24, 发布年份 2023 | |
来源: Springer | |
【 摘 要 】
BackgroundThe clinical symptoms of invasive fungal infections (IFI) are nonspecific, and early clinical diagnosis is challenging, resulting in high mortality rates. This study reports the development of a novel aptamer-G-quadruplex/hemin self-assembling color system (AGSCS) based on (1 → 3)-β-D-glucans’ detection for rapid, specific and visual diagnosis of IFI.MethodsWe screened high affinity and specificity ssDNA aptamers binding to (1 → 3)-β-D-glucans, the main components of cell wall from Candida albicans via Systematic Evolution of Ligands by EXponential enrichment. Next, a comparison of diagnostic efficiency of AGSCS and the (1 → 3)-β-D-glucans assay (“G test”) with regard to predicting IFI in 198 clinical serum samples was done.ResultsWater-soluble (1 → 3)-β-D-glucans were successfully isolated from C. albicans ATCC 10,231 strain, and these low degree of polymerization glucans (< 1.7 kD) were targeted for aptamer screening with the complementary sequences of G-quadruplex. Six high affinity single stranded DNA aptamers (A1, A2, A3, A4, A5 and A6) were found. The linear detection range for (1 → 3)-β-D-glucans stretched from 1.6 pg/mL to 400 pg/mL on a microplate reader, and the detection limit was 3.125 pg/mL using naked eye observation. Using a microplate reader, the sensitivity and specificity of AGSCS for the diagnosis of IFI were 92.68% and 89.65%, respectively, which was higher than that of the G test.ConclusionThis newly developed visual diagnostic method for detecting IFI showed promising results and is expected to be developed as a point-of-care testing kit to enable quick and cost effective diagnosis of IFI in the future.
【 授权许可】
CC BY
© The Author(s) 2023
【 预 览 】
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【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]
- [33]
- [34]
- [35]
- [36]
- [37]
- [38]
- [39]
- [40]
- [41]
- [42]
- [43]
- [44]
- [45]
- [46]