A novel aptamer-G-quadruplex/hemin self-assembling color system: rapid visual diagnosis of invasive fungal infections

Annals of Clinical Microbiology and Antimicrobials

Research

Yueling Xiong¹  Xiaolei Tang¹  Feng Hu²  Ying Hua³  Fan Su⁴  Yufeng Wen⁴  Jian Hu⁴  Xia Ren⁴ 

[1] Centre of Translational Medicine and Vascular Disease Research Center, The Second Affiliated Hospital of Wannan Medical College, Kangfu Road 10#, Jinghu District, 241000, Wuhu, Anhui, China;Department of Blood Transfusion, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), 241000, Wuhu, Anhui, China;School of Nursing, Wannan Medical College, 241000, Wuhu, Anhui, China;School of Public Health, Wannan Medical College, No.22, Wenchang Xi Road, 241002, Wuhu, Anhui, China;

关键词: Invasive fungal infections (IFI);    Aptamers;    Systematic Evolution of Ligands by EXponential enrichment;    Self-assembling;    Visual diagnosis;    G-quadruplex;   

DOI  :  10.1186/s12941-023-00570-6

 received in 2022-10-25, accepted in 2023-02-24,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe clinical symptoms of invasive fungal infections (IFI) are nonspecific, and early clinical diagnosis is challenging, resulting in high mortality rates. This study reports the development of a novel aptamer-G-quadruplex/hemin self-assembling color system (AGSCS) based on (1 → 3)-β-D-glucans’ detection for rapid, specific and visual diagnosis of IFI.MethodsWe screened high affinity and specificity ssDNA aptamers binding to (1 → 3)-β-D-glucans, the main components of cell wall from Candida albicans via Systematic Evolution of Ligands by EXponential enrichment. Next, a comparison of diagnostic efficiency of AGSCS and the (1 → 3)-β-D-glucans assay (“G test”) with regard to predicting IFI in 198 clinical serum samples was done.ResultsWater-soluble (1 → 3)-β-D-glucans were successfully isolated from C. albicans ATCC 10,231 strain, and these low degree of polymerization glucans (< 1.7 kD) were targeted for aptamer screening with the complementary sequences of G-quadruplex. Six high affinity single stranded DNA aptamers (A1, A2, A3, A4, A5 and A6) were found. The linear detection range for (1 → 3)-β-D-glucans stretched from 1.6 pg/mL to 400 pg/mL on a microplate reader, and the detection limit was 3.125 pg/mL using naked eye observation. Using a microplate reader, the sensitivity and specificity of AGSCS for the diagnosis of IFI were 92.68% and 89.65%, respectively, which was higher than that of the G test.ConclusionThis newly developed visual diagnostic method for detecting IFI showed promising results and is expected to be developed as a point-of-care testing kit to enable quick and cost effective diagnosis of IFI in the future.

【 授权许可】

CC BY   
© The Author(s) 2023

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