A self-aggregating peptide: implications for the development of thermostable vaccine candidates

BMC Biotechnology

J. Iván Sánchez-Betancourt¹  Luis Vaca²  Carlos Bastián-Eugenio²  Alicia Sampieri²  Adolfo Cruz-Reséndiz²  Jesús Zepeda-Cervantes²  Goar Gevorkian³  Gonzalo Acero³ 

[1] Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México;Instituto de Fisiología Celular, Universidad Nacional Autónoma de México;Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México;

关键词: Vaccines;    Particles;    Self-assembling;    Thermostable;    Immunology;   

DOI  :  10.1186/s12896-019-0592-9

来源: DOAJ

【 摘 要 】

Abstract Background The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH (1–110) from polyhedrin self-aggregates and incorporates foreign proteins to form particles. We have proposed that this peptide can be used as an antigen carrying system for vaccines. However, the immune response generated by the antigen fused to the peptide has not been fully characterized. In addition, the adjuvant effect and thermostability of the particles has not been evaluated. Results In the present study we demonstrate the use of a system developed to generate nano and microparticles carrying as a fusion protein peptides or proteins of interest to be used as vaccines. These particles are purified easily by centrifugation. Immunization of animals with the particles in the absence of adjuvant result in a robust and long-lasting immune response. Proteins contained inside the particles are maintained for over 1 year at ambient temperature, preserving their immunological properties. Conclusion The rapid and efficient production of the particles in addition to the robust immune response they generate position this system as an excellent method for the rapid response against emerging diseases. The thermostability conferred by the particle system facilitates the distribution of the vaccines in developing countries or areas with no electricity.

【 授权许可】

Unknown