Exploring the impact of glutathione S -transferase (GST)-based metabolic resistance to insecticide on vector competence of Anopheles funestus for Plasmodium falciparum

Wellcome Open Research

article

Cyrille Ndo¹  Edmond Kopya⁴  Helen Irving³  Charles Wondji¹ 

[1] Department of parasitology, Centre for Research in Infectious Disease;Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala;Vector Group, Liverpool School of Tropical Medicine;Institut de recherche de Yaoundé ,(IRY), Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale;Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaoundé I

关键词: Malaria;    Insecticide resistance;    Anopheles funestus;    Plasmodium falciparum;    metabolic resistance;    GSTe2;   

DOI  :  10.12688/wellcomeopenres.15061.2

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background: Malaria control heavily relies on insecticide-based interventions against mosquito vectors. However, the increasing spread of insecticide resistance is a major threat. The extent to which such resistance, notably metabolic resistance, influences the development of thePlasmodium parasite and its impact on overall malaria transmission remains poorly characterized. Here, we investigated whether glutathione S-transferase-based resistance could influencePlasmodium falciparum development inAnopheles funestus.Methods: Anopheles funestus females were infected withP. falciparum gametocytes and midguts were dissected at day 7 post infection for detection/quantification of oocysts. Infection parameters were compared between individuals with different L119F-GSTe2 genotypes, and the polymorphism of the GSTe2 gene was analyzed in infected and uninfected mosquito groups.Results: Overall, 403An. funestus  mosquitoes were dissected and genotyped. The frequency of the L119F-GSTe2 resistance allele was significantly higher in non-infected (55.88%) compared to infected (40.99%) mosquitoes (Fisher's exact test, P<0.0001). Prevalence of infection was significantly higher in heterozygous and homozygous susceptible genotypes (P<0.001). However, homozygous resistant and heterozygous mosquitoes exhibited significantly higher infection intensity (P<0.01). No association was observed between the GSTe2 polymorphism and the infection status of mosquitoes.Conclusion: Altogether, these results suggest that GSTe2-based metabolic resistance may affect the vectorial competence of resistantAn. funestus mosquitoes toP. falciparum infection, by possibly increasing its permissiveness toPlasmodium infection.

【 授权许可】

CC BY