B cell, CD8 + T cell and gamma delta T cell infiltration alters alveolar immune cell homeostasis in HIV-infected Malawian adults

Wellcome Open Research

article

Andrew Mwale¹  Annemarie Hummel¹  Leonard Mvaya¹  Raphael Kamng'ona¹  Elizabeth Chimbayo¹  Joseph Phiri¹  Rose Malamba¹  Anstead Kankwatira¹  Henry C Mwandumba¹  Kondwani C Jambo¹ 

[1] Malawi-Liverpool-Wellcome Trust Clinical Research Programme, University of Malawi College of Medicine;Department of Clinical Sciences, Liverpool School of Tropical Medicine

关键词: BAL;    HIV;    B cell;    CD8 T cell;    gamma-delta T cells;    CD4 T cell;    alveolitis;    adult;   

DOI  :  10.12688/wellcomeopenres.12869.3

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background: HIV infection is associated with increased risk to lower respiratory tract infections (LRTI). However, the impact of HIV infection on immune cell populations in the lung is not well defined. We sought to comprehensively characterise the impact of HIV infection on immune cell populations in the lung.Methods: Twenty HIV-uninfected controls and 17 HIV-1 infected ART-naïve adults were recruited from Queen Elizabeth Central Hospital, Malawi. Immunophenotyping of lymphocyte and myeloid cell populations was done on bronchoalveolar lavage fluid and peripheral blood cells.Results: We found that the numbers of CD8+ T cells, B cells and gamma delta T cells were higher in BAL fluid of HIV-infected adults compared to HIV-uninfected controls (all p<0.05). In contrast, there was no difference in the numbers of alveolar CD4+ T cells in HIV-infected adults compared to HIV-uninfected controls (p=0.7065). Intermediate monocytes were the predominant monocyte subset in BAL fluid (HIV-, 63%; HIV+ 81%), while the numbers of classical monocytes was lower in HIV-infected individuals compared to HIV-uninfected adults (1 × 105 vs. 2.8 × 105 cells/100ml of BAL fluid, p=0.0001). The proportions of alveolar macrophages and myeloid dendritic cells was lower in HIV-infected adults compared to HIV-uninfected controls (all p<0.05).Conclusions: Chronic HIV infection is associated with broad alteration of immune cell populations in the lung, but does not lead to massive depletion of alveolar CD4+ T cells. Disruption of alveolar immune cell homeostasis likely explains in part the susceptibility for LRTIs in HIV-infected adults.

【 授权许可】

CC BY