PARV4 prevalence, phylogeny, immunology and coinfection with HIV, HBV and HCV in a multicentre African cohort

Wellcome Open Research

article

Colin P. Sharp¹  William F. Gregory¹  Louise Hattingh³  Amna Malik⁴  Emily Adland⁴  Samantha Daniels³  Anriette van Zyl³  Jonathan M. Carlson⁵  Susan Wareing⁶  Anthony Ogwu⁷  Roger Shapiro⁷  Lynn Riddell⁸  Fabian Chen⁹  Thumbi Ndung'u^1,10  Philip J.R. Goulder⁴  Paul Klenerman⁶  Peter Simmonds^1,11  Pieter Jooste³  Philippa C. Matthews⁶ 

[1] Roslin Institute, University of Edinburgh;Edinburgh Genomics, University of Edinburgh;Kimberley Hospital;Department of Paediatrics, University of Oxford;Microsoft Research;Department of Microbiology and Infectious Diseases, Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital;Botswana Harvard AIDS Institute Partnership;Northampton General Hospital NHS Trust;Royal Berkshire Hospital;HIV Pathogenesis Program, Nelson R. Mandela School of Medicine, University of KwaZulu-Natal;Nuffield Department of Medicine, University of Oxford;NIHR Biomedical Research Centre, John Radcliffe Hospital

关键词: PARV4;    parvovirus;    Africa;    co-infection;    epidemiology;    HBV;    HCV;    HIV;   

DOI  :  10.12688/wellcomeopenres.11135.1

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background: The seroprevalence of human parvovirus-4 (PARV4) varies considerably by region. In sub-Saharan Africa, seroprevalence is high in the general population, but little is known about the transmission routes or the prevalence of coinfection with blood-borne viruses, HBV, HCV and HIV. Methods: To further explore the characteristics of PARV4 in this setting, with a particular focus on the prevalence and significance of coinfection, we screened a cohort of 695 individuals recruited from Durban and Kimberley (South Africa) and Gaborone (Botswana) for PARV4 IgG and DNA, as well as documenting HIV, HBV and HCV status. Results: Within these cohorts, 69% of subjects were HIV-positive. We identified no cases of HCV by PCR, but 7.4% were positive for HBsAg. PARV4 IgG was positive in 42%; seroprevalence was higher in adults (69%) compared to children (21%) (p<0.0001) and in HIV-positive (52%) compared to HIV-negative individuals (24%) (p<0.0001), but there was no association with HBsAg status. We developed an on-line tool to allow visualization of coinfection data (https://purl.oclc.org/coinfection-viz). We identified five subjects who were PCR-positive for PARV4 genotype-3.Ex vivo CD8+ T cell responses spanned the entire PARV4 proteome and we propose a novel HLA-B*57:03-restricted epitope within the NS protein. Conclusions: This characterisation of PARV4 infection provides enhanced insights into the epidemiology of infection and co-infection in African cohorts, and provides the foundations for planning further focused studies to elucidate transmission pathways, immune responses, and the clinical significance of this organism.

【 授权许可】

CC BY