The Effects of Maternal Exposure Riboflavin to Prevent Uterus Arsenic Damage in Offspring Rats

Journal of Human, Environment, and Health Promotion

article

Parichehr Nouri¹  Ali Olfati² 

[1]Department of Midwifery, School of Nursing and Midwifery, Kermanshah University of Medical Sciences

[2]Researcher, Young Researchers and Elites Club, Kermanshah Branch, Islamic Azad University

关键词: As2O3;    Inflammation;    Puberty;    Vitamin B2;   

DOI  :  10.52547/jhehp.7.4.182

来源: Journal of Human Environment and Health Promotion

【 摘 要 】

Background: In this study, the efficacy of riboflavin (VB2) in preventing uterus As2O3 damage was investigated for the first time in the literature. Methods: The rats received 40 μg LHRHa for estrus synchronization. 48 pregnant Wistar rats were included in the study. Four groups were formed with 7 rats in each group: Sham, 1.5 mg arsenic trioxide (As2O3/L) alone or in combination with VB2 (20 and 40 mg/L) in drinking water (for 21 days continuously). Moreover, similar to maternal generation treatment the F1-female generation was arranged (for 35 days continuously until puberty). Results: Based on the results, As2O3 reduced body weight and feed intake (P < 0.05). Furthermore, the serum malondialdehyde levels in the As2O3 group were significantly higher than that of the control group (P < 0.05). At the same time, the total antioxidative status and the activities of glutathione peroxidase, superoxide dismutase, and catalase were reduced (P < 0.05). Meanwhile, As2O3 remarkably increased the inflammatory markers production [interleukin 6 and C-reactive protein] (P < 0.05). As2O3 administration induced uterus apoptosis-related genes by upregulating caspase-3, iNOS, and Bax genes and downregulating Bcl-2 gene of pubertal F1-female rats (P < 0.05). Conclusion: Our observation indicated that VB2 therapy is potentially an effective strategy to modify the detrimental effects of As2O3 in pubertal F1-female rats via suppresses oxidative damages.

【 授权许可】

Unknown